Cellular Respiration

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Cellular Respiration

In this lab the effects of different substrates on the rate of cellular respiration is being put to a test which is a very interesting experiment. The three major substrate solutions being used for this experiment are glucose, maltose, and alanine. The issues this experiment addresses are cellular respiration occur in different stages which are glycolysis, citric acid cycle, and fermentation. In this lab the experiment determines the effect of different substrates on rate of cellular respiration. Maltose would result in the greatest rate of cellular respiration the rationale is because it gives more carbon than others. Glucose would be a good alternate and result in the second best rate of cellular respiration the rationale is because it has six carbons. Alanine would result in the worst rate of cellular respiration the rationale is because it has the least carbon. The materials used for this experiment are test tubes, test tube racks, pipettes 10 ml, propipettes, beakers 100 ml, distilled water the substrate solutions used were glucose, maltose, and alanine also yeast suspension. The temperature bath was at 37 degrees and metric ruler was used for measurements of the air bubbles. - Preparation of an appropriate control

-3.3 ml to 3.3 ml ratio of distilled water to substrate
-3.3 plus ml of yeast to fill test tubes
-Incubation temperature 37 degrees
-15 minute measurement (mm) intervals
-Calculation of actual CO2 production
-Mix three tubes
-Incubate mix control
-Add yeast to the top
-Invert test tubes
-Measure out bubbles with metric ruler
-Glucose and maltose
The conclusion of this experiment was that glucose was the greatest rate of the cellular respiration the rationale is because it is completely broken down to CO2 and most of ATP is generated by glucose. The simplest sugars work the best because energy does not have to expend to break them down into usable forms. The second best rate of...
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