After completing the exercise, I was able to:
a) Prepare the specimens for staining.
b) Observe and identify the unique and typical structures of the microscopic world. c) Identify and use different stains for different types of organelles.
Cell is the simplest unit of life as we see it now. All organisms are made from cells. It is first explained by Robert Hooke in the year 1665 and it is now known to be of almost universal occurrence in organisms.
Cell theory refers to the idea that cells are the basic unit of structure in every living thing. Development of this theory during the mid 1600s was made possible by advances in microscopy. In the hierarchy of biological organization, the cell is the simplest collection of matter that can live. There are diverse forms of life that exist as single-celled organism or complex organism. Though in general, most cells are similar, they still show considerable diversity especially in their contents, shape and function. However, with the creation of microscope, scientists and researchers are able to study the specimen of a cell and identify its different structure.
Big structures such as the cell wall, cell membrane and nucleus could be seen under the light microscope. Nevertheless, some smaller structures are still too small to be identified without first staining it. Stains can be used to enhance contrast in microscopy image. The choices of stain are important because different stains react or concentrate in different parts of a cell or tissue. This can be seen when acetocarmine stains the nucleus and its contents; iodine solution stains starch grains. Through this experiment, we will be able to determine the structures in the cell.
Results and Discussion
I. Epidermal Cells of Plants
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The diagram drawn is the upper layer of the cell of the Rheo Discolours leaf. From the diagram, we can see that the cells structured together, that is it...