In order to maintain structure, individual cells are responsible for breaking down molecules to receive adenosine triphosphate (ATP) for energy. This is action is referred to as cellular respiration; cell respiration uses nutrient molecules and oxygen to release a product of water and carbon dioxide. The equation for cellular respiration is C6H12O6 + 6O2 → 6CO2 + 6H2O + Energy (as ATP). Cellular respiration has several different ways that it is measured. For instance, since respiration is exergonic, one can measure by temperature. Additionally, measuring oxygen consumption or carbon dioxide production is another reliable method. In this lab, the rate of oxygen consumption in germinating and non-germinating peas will be calculated using two different temperatures.
First, in order to calculate the gas, the Ideal Gas Law formula PV=nrT is required. For the equation, P is the pressure of gas. V is the volume of the gas. T represents the temperature.
If the respirometer with germinating peas have a larger consumption of oxygen, therefore, having a larger amount of Carbon dioxide than the other two respirometers with just beads or beads and dry peas. Materials:
The materials needed for the lab are the following: 50 germinating pea seeds, 50 dry seeds, 100 plastic beads, three respirometer vials, weights for vials, three plastic stoppers, 1 mL graduated pipets, sealant, absorbent cotton, nonabsorbent cotton, one round stick, three pieces of paper towel, marking pen, water bath, ice, 100 mL graduated cylinder, thermometer, masking tape, stopwatch or clock, and dropper bottle of 15% KOH. Method:
The following procedures split into three parts. In the part one, one must label three paper towels as follows: 1a, 2a, 3a. The numbers correspond to the respirometers of the same numbers. First, fill a graduated cylinder with 20 mL of water. Painfully, count out 25 germinating seeds and place them into a graduated cylinder. Record the total volume of...
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