Catalase Reactions

Topics: Oxygen, Enzyme, Hydrogen peroxide Pages: 2 (586 words) Published: February 11, 2013
The aim of this experiment was to find out how the pH level of hydrogen peroxide affects the Catalase. Based on the results gotten from the experiment, it shows that Catalase works the best at the pH of 4. The oxygen levels at the pH of 4 are greater than at the pH of 5.5, 6, and 8. Also the distances the Oxygen foam rose are also higher than at the other pHs. For example, for Trial #1 for pH 4, the oxygen level was 0.7 and the height was 1.7 cm while for Trial #1 at pH of 6, the oxygen level was 0.25 and the height was 0.5 cm. The heights of the Oxygen foam in all trials were measured after 10 seconds after the liver was added to the hydrogen peroxide. The data table also shows that the Catalase worked best at the pH of 4. The highest height the oxygen foam rose was 1.7 cm, which is when the pH was 4. The second highest height was 1.5 cm, which is also when the pH was 4.

Catalase also worked well at the pH of 8 because the oxygen foam rose to 1.3 cm but it did not work as good as at the pH of 4. The graphs for the oxygen levels show that the oxygen level was supposed to rise after the liver was added to the hydrogen peroxide. For example, in graph of Run #3 (Trial #1 & #2 at pH of 4) show the rise in the oxygen level after 60 seconds. It also shows the same pattern in Run #4 (Trial 1 & 2 at pH of 8) after 60 seconds. The pattern does not show up in Run #1 (Trial 1 & 2 at pH of 5.5) and Run #2 (Trial 1 & 2 at pH of 6). This is because the Catalase was not working at the pHs of 5.5 and 6.

There are a lot of downsides in this lab. First, the pH probes and the Oxygen sensors might not have been calibrated. However, the pH probes were tested by measuring the pH of water and other liquids whose pHs were known. But the Oxygen Gas sensor might not have been correct because it was a bit hard to know if they were working correctly because in the trials at pH of 5.5 and 6, the oxygen gas sensor showed the same number and changed only...
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