Chromatography is a physical method of separation in which the components to be separated are distributed between two phases, one of which is stationary (stationary phase) while the other (the mobile phase) moves in a definite direction. It separates the analyte to be measured from other molecules in the mixture and allows it to be isolated.
Chromatography may be preparative or analytical. Preparative chromatography seeks to separate the components of a mixture for further use (and is thus a form of purification). Analytical chromatography normally operates with smaller amounts of material and seeks to measure the relative proportions of analytes in a mixture.
In modern medicine, specialist or scientists make use of this chromatography to examine a mixture, its components, and their relations to one another or to analyze. They also make use of this to separate the components in order to isolate one of interest for further study or to purify. Another is to determine the identity of a mixture or components based on known components or to identify. Scientist also makes use of chromatography to determine the amount of the mixture or the components present in the sample or quantification.
Chromatography works because the components of the mixture will differ in how much they "stick" to things: to each other, and to other substances. For example, some of the components of the ink will stick more tightly to the paper fibers. They will spend less time in the water as it moves along the paper fibers, and thus they will not travel very far. Other components of the ink will stick less tightly to the paper fibers. They will spend more time in the water as it moves along the paper fibers, and thus they will travel farther through the paper. Other materials than paper and water can be used for chromatography, but in each chromatography apparatus there is generally a stationary phase and a mobile phase. In paper chromatography, the paper is the stationary phase, and...
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