Cancer Gene Detection
Cancer Gene Detection
11/06/12
Laboratory Report
Objective(s) – The purpose of this experiment is to help students gain an understanding of p53 tumor suppressor genes and its role in familial cancers. Also, we evaluated p53 in cancer.
Hypothesis – If the DNA has bits and particles, which are p53 hotspots being cut, than that means that cancer has been detected.
Techniques & Skills – The skills that are required for this laboratory experiment is that we must exercise extreme caution when working with equipment that is used in conjunction with the heating and/or melting of reagents. Also we must have the ability to use the pipet efficiently and effectively because we will be extracting substances that need to be handled with care. The casting trays (bed) that is being handled needs to be used with care and skill because it should not be messed with but used in the proper sense.
Results & Observations – During this experiment we observed that the DNA samples moved left about 3-4 centimeters while electrolytes where being passed through them. This reason was because the substances were being tested if they were being broken or not. After about 20 minutes, we took the substances to the lab and placed it into a molecular imaging machine that read the substances. To conclude the experiment we observed that the p53 hotspots were cut, meaning that cancer has been detected in Valerie. In the image that is stapled to the back of this report, if we look carefully we can see where the controlled subject but also the experimental. Then we see that there is DNA that has been broken. That is the sign of cancer.
Study Questions:
1. Tumor suppressors: Are what stops a cell from dividing and developing tumors Oncogenes: Genes that in certain circumstances transforms a cell into a tumor cell
2. Hotpots in p53 protein structures create restriction sites where the DNA is cut.
3. Because Valerie’s tumor DNA sample was just some fragments of the cancer but was not
11/06/12
Laboratory Report
Objective(s) – The purpose of this experiment is to help students gain an understanding of p53 tumor suppressor genes and its role in familial cancers. Also, we evaluated p53 in cancer.
Hypothesis – If the DNA has bits and particles, which are p53 hotspots being cut, than that means that cancer has been detected.
Techniques & Skills – The skills that are required for this laboratory experiment is that we must exercise extreme caution when working with equipment that is used in conjunction with the heating and/or melting of reagents. Also we must have the ability to use the pipet efficiently and effectively because we will be extracting substances that need to be handled with care. The casting trays (bed) that is being handled needs to be used with care and skill because it should not be messed with but used in the proper sense.
Results & Observations – During this experiment we observed that the DNA samples moved left about 3-4 centimeters while electrolytes where being passed through them. This reason was because the substances were being tested if they were being broken or not. After about 20 minutes, we took the substances to the lab and placed it into a molecular imaging machine that read the substances. To conclude the experiment we observed that the p53 hotspots were cut, meaning that cancer has been detected in Valerie. In the image that is stapled to the back of this report, if we look carefully we can see where the controlled subject but also the experimental. Then we see that there is DNA that has been broken. That is the sign of cancer.
Study Questions:
1. Tumor suppressors: Are what stops a cell from dividing and developing tumors Oncogenes: Genes that in certain circumstances transforms a cell into a tumor cell
2. Hotpots in p53 protein structures create restriction sites where the DNA is cut.
3. Because Valerie’s tumor DNA sample was just some fragments of the cancer but was not