Chapter 20 Sample Questions: MULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers the question.
1) It is theoretically possible for a gene from any organism to function in any other organism. Why is this possible? A) All organisms have ribosomes. B) All organisms have the same genetic code. C) All organisms are made up of cells. D) All organisms have similar nuclei. E) All organisms have transfer RNA. 2) One important approach to gene cloning uses A) noncoding nucleotide sequences. B) plasmids. C) whole chromosomes. D) bacteria. E) B and D above. 3) Current applications of gene cloning include A) A and C only. B) A, B, and C. C) cleaning up toxic waste. D) instilling pest resistance in plants. E) manufacturing human growth hormone. 4) Plasmids are important in biotechnology because they are A) a vehicle for the insertion of recombinant DNA into bacteria. B) surfaces for respiratory processes in bacteria. C) recognition sites on recombinant DNA strands. D) surfaces for protein synthesis in eukaryotic recombinants. E) proviruses incorporated into the host DNA. 5) If you discovered a bacterial cell that contained no restriction enzymes, which of the following would you expect to happen? A) The cell would create incomplete plasmids. B) The cell would be unable to replicate its DNA. C) The cell would become an obligate parasite. D) The cell would be easily infected and lysed by bacteriophages. E) Both A and D would occur. 6) Assume that you are trying to insert a gene into a plasmid and someone gives you a preparation of DNA cut with restriction enzyme X. The gene you wish to insert has sites on both ends for cutting by restriction enzyme Y. You have a plasmid with a single site for Y, but not for
X. Your strategy should be to A) cut the plasmid with restriction enzyme X and insert the fragments cut with Y into the plasmid. B) cut the plasmid with enzyme X and then insert the gene into the plasmid. C) cut the DNA again with...
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