Biology Lab Report

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  • Topic: Ion, Osmosis, Osmotic pressure
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  • Published : February 19, 2013
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DIFFUSION AND OSMOSIS

Prepared by : Puteri Ayu Kirana binti Ahmad Jayaputera
( Lab Partner : Ashami Athukorala )

AIM :

To study the phenomenon of diffusion and osmosis

PROCEDURE :
Please refer to the laboratory manual

RESULT :

A) Diffusion

The petri dish of agar is then placed in a dark place. After two hours, the results are shown in the picture below :

The color results of the reaction is shown below :-

Band| Reaction| Color|
AB| AgCl| White|
AC| AgBr| None|
AD| Ag3Fe(CN)6| Yellow|

1. The distance between band A and B is 1.3cm.
2. The distance between band A and C is none.
3. The distance between band A and D is 2.6cm.
4. The farthest band from hole A is D, thus its ion is the slowest migrating species. 5. The closest band from hole A is B, thus its ion is the fastest-migrating species. 6. The ions in hole B are expected to move the fastest.

7. The ions in hole D are expected to move the slowest.

B) Osmosis

(a) Osmosis in a Model Cell
The results at the end of the experiment is shown below :

The Solution A turns blue-black and the dialysis tubing becomes bigger at the end of the experiment.

(b) Osmosis in a Living Cell

At the beginning of the experiment, the onion cell looks like as below :

After adding sodium chloride solution, the cytoplasm of the onion cell shrinked away from the cell wall as shown below :

After adding a few drops of deionized water, the cytoplasm of the onion cell pushed towards the cell wall as shown below :

DISCUSSION :

A) Diffusion

1. Colored bands are formed around hole B, C and D at the end of the experiment. 2. Molecular masses of the ions in the three holes can be ranked C1-<Br-<Fe(CN)63-. 3. The larger the molecular mass of the ion, the slower the ion will migrate. 4. The smaller the molecular mass of the ion, the faster the ion will migrate. 5. The ions that have the least...
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