Biology Lab

Topics: Chromatography, Analytical chemistry, Gas chromatography Pages: 247 (79203 words) Published: January 18, 2013
TEXT BOOK OF

BIOCHEMISTRY
For 2nd year Medical Laboratory Technician Course
(INTERMEDIATE VOCATIONAL)

Author :

S. Srinivasa Rao, B.Pharmacy,
Jr. Lecturer in M.L.T., Alluri Sri Rama Raju Govt. Junior College, Shanthi Nagar, Khammam.

Editor :

S. Kamalakar Rao, M. Pharmacy
Senior Lecturer in Pharmacy, Department of Biotechnology, Govt. institute for post diploma courses in engineering and technology, Ramanthapur,Hyderabad.

INDEX
Chapter I. Page No. Instrumental methods of Biochemical analysis ..................... 1 1. Flame Photometry ................................................................... 1 2. Fluorimetry ........................................................................... 10 3. Nephelometry ....................................................................... 12 4. Basic principles and applications of Potentiometry .................. 16 II. Separation techniques ............................................................ 25 1. Chromatography ................................................................... 25 2. Electrophoresis ..................................................................... 53 III. Immunoassays ........................................................................ 70 1. Radio immunoassays ............................................................. 70 2. Enzyme linked immunoassays ................................................ 89 IV. Metabolism ............................................................................. 94 1. Carbohydrate metabolism...................................................... 95 2. Lipid metabolism ................................................................. 101 3. Protein metabolism .............................................................. 104 V. Titrimetric methods of quantitative determination, Preparation of varioussolutions used in Titrimetric analysis .............. 109 VI. Liver function tests .............................................................. 153 1. Basic concepts including normal and abnormal bilirubin metabolism .............................................................. 153 2. Classification ............................................................................. 157 3. Serum Bilirubin determination .............................................. 158

4. Vandenbergh test ................................................................. 166 5. Total proteins and A/G ratio.............................................. 167 6. Enzyme estimations as LFT ................................................. 173 VII.Renal function tests ............................................................. 178 1. Basic concepts and Classification........................................ 178 2.Clearance tests..................................................................... 183 3.Concentration and dilution tests ............................................ 186 4.Urine examination in assessing kidney function....................... 187 VIII.Gastric function tests ......................................................... 217 1. Basic concepts and introduction........................................... 217 2. Techniques of different tests including Tubeless Gastric analysis ..................................................... 222 IX.Thyroid function tests ........................................................... 233 1. Basic concepts .................................................................... 233 2. Estimations of various thyroid hormones, their interpretations ............................................................. 235 3. Recent methods of Thyroid function tests ............................. 236 X.Pancreatic function tests ........................................................ 239 1. Basic concepts and introduction........................................ 239 2. Various tests done and methods including serum amylase determination ......................................................
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