Lab 5: culture transfer instruments, techniques and isolation and maintenance of pure cultures.
Background: Pure cultures of multicellular organisms are often more easily isolated by simply picking out a single individual to initiate a culture. This is a useful technique for pure culture of fungi, multicellular algae. To aseptically transfer sufficient bacteria to an inoculated agar slant to start a new pure culture Aim: the aim of this experiment is to correctly use the pipette, inoculating loop and needle and use technique to remove and transfer bacteria for subculturing. And prepare stock culture. Methods: the procedure for isolation of pure culture and the maintaince we label five tubes of each type, them we do the aseptic technique, select a well isolated colony for the bacteria using an inoculating loop Then prepare a slant culture, broth culture, deep culture and agar culture. Results: we examined the pure cultures for bacterial distribution and color growth. We found some are pure some are cloudy.
Introduction: Once discrete, well-separated colonies develop on the surface of the streak plate, selected ones may be picked up with an inoculating needle and transferred to separate culture tubes such as tryptic soy agar slants (the type of agar will depend on the microorganism). Where possible, bacteria from the center of a colony are transferred, because the center is less likely to be contaminated than the edges. Each slant now represents the growth of a single species of microorganism and is called a pure or stock culture.
Material and methods:
Strains: E. coli 6 agar plates, B.subtilis 6 agar plates. Madia: LB agar slant, LB broth, LB deep, LB agar plate. Materials: vortex mixter, incolating loop, Bunsen burner, blow out pipette, wax pencil. The procedure for isolation of pure culture and the maintenance we label five tubes of each type, then we do the aseptic technique, Select a well isolated colony for the bacteria using an...
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