Biology Enzyme Lab

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LAB: Substrate Concentration Affecting the Rate of Enzyme Activity:

Through the Experiment of Beef Liver Puree and Hydrogen Peroxide

Research Question

Does different amount of substrate affect the rate of enzyme activities?

Purpose

To examine how different types of concentration (Hydrogen Peroxide) affect the rate of enzyme activity.

Hypothesis

We believe that if there is more substrate concentrated, then there will be an increase in the rate of enzyme activity. This is because we assume the more substrate an enzyme gets the chance to interact with, the higher the probability of a lot of the enzyme working, and the more enzyme working leads to the increase in enzyme activity.

Variables

Independent Variable: Concentration of substrate (Hydrogen peroxide)

Dependent Variable: Rate of enzyme reaction

Controlled Variable: Distance, amount of enzyme of paper disc, temperature which experiment took place, everything else basically.

Data Collection & Processing

|Concentration of Hydrogen Peroxide (%) |Time (seconds) |Distance (cm) | |100 |2.5 |9.9 | |100 |2.8 |9.9 | |100 |2.4 |9.8 | |100 |2.8 |9.9 | |100 |2.6 |10.0 | |75 |3.9 |9.9 | |75 |3.6 |9.7 | |75 |3.8 |9.8 | |75 |4.0 |9.8 | |75 |3.5 |9.8 | |50 |4.5 |9.9 | |50 |4.9 |10.2 | |50 |4.9 |9.8 | |50 |4.7 |10.1 | |50 |4.9 |9.9 | |25 |5.3 |10.1 | |25 |5.6 |10.0 | |25 |5.2 |10.0 | |25 |5.2 |9.9 | |25 |5.2...
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