Biol 111 lab report water
What organic molecule can be detected with Lugol’s?
By using the Lugol’s iodine test, we can detect the presence of starches.
Why put Lugol’s in the beaker and not the bag?
When we put the Lugol’s regent (I2Kl) in the beaker, we can observe the effects of osmosis as the Lugol’s regent (I2Kl) solution on the outside of the beaker permeates the intestine (Dialysis tubing) inducing a chemical reaction that will turn the liquid in the intestine (Dialysis tubing) blue-black.
If the amylase successfully digests the starch, what results would you expect?
If the amylase successfully digests the starch, I would expect a no color change occur as well as a presence of glucose in the intestine (Dialysis tubing).
If the amylase only partly digests the starch, what would you expect to happen?
If the amylase only partly digests the starch, I would expect to see a faint color change as well as a small presence of glucose inside the intestine.
If the amylase fails to digest the starch, what results would you expect?
If the amylase fails to digest the starch, I would expect the solution inside the intestine (Dialysis tubing) to change color to blue-black due to the chemical action that will occur when the Lugol’s regent (I2Kl) permeates through the intestine (Dialysis tubing).
Why is it necessary to have a control for this experiment?
By having a control in this experiment, we can see the changes with the presence of amylase. Lugol’s regent (I2Kl) changes color in the presence of starch, which is the control group that we have setup that demonstrates what would happen naturally, with starch and Lugol’s regent (I2Kl). The control group will have a distinct comparison available for us. As amylase, an enzyme that catalyzes the breakdown of the starch into glucose, which will have no reaction with Lugol’s regent (I2Kl) as well as no color change.
Were starch molecules able to cross the membrane?
No, the starch molecules were not able to cross the membrane,
By using the Lugol’s iodine test, we can detect the presence of starches.
Why put Lugol’s in the beaker and not the bag?
When we put the Lugol’s regent (I2Kl) in the beaker, we can observe the effects of osmosis as the Lugol’s regent (I2Kl) solution on the outside of the beaker permeates the intestine (Dialysis tubing) inducing a chemical reaction that will turn the liquid in the intestine (Dialysis tubing) blue-black.
If the amylase successfully digests the starch, what results would you expect?
If the amylase successfully digests the starch, I would expect a no color change occur as well as a presence of glucose in the intestine (Dialysis tubing).
If the amylase only partly digests the starch, what would you expect to happen?
If the amylase only partly digests the starch, I would expect to see a faint color change as well as a small presence of glucose inside the intestine.
If the amylase fails to digest the starch, what results would you expect?
If the amylase fails to digest the starch, I would expect the solution inside the intestine (Dialysis tubing) to change color to blue-black due to the chemical action that will occur when the Lugol’s regent (I2Kl) permeates through the intestine (Dialysis tubing).
Why is it necessary to have a control for this experiment?
By having a control in this experiment, we can see the changes with the presence of amylase. Lugol’s regent (I2Kl) changes color in the presence of starch, which is the control group that we have setup that demonstrates what would happen naturally, with starch and Lugol’s regent (I2Kl). The control group will have a distinct comparison available for us. As amylase, an enzyme that catalyzes the breakdown of the starch into glucose, which will have no reaction with Lugol’s regent (I2Kl) as well as no color change.
Were starch molecules able to cross the membrane?
No, the starch molecules were not able to cross the membrane,