There have been several studies on the biodegradation of DDT but most of these involved DDT in water. The researchers deem that it is also important to investigate on the degradation of DDT in water because due to run-off, there have been accumulations of DDT in the bodies of water.
The objectives of the study are to determine the extent of DDT degradation; determine the effects of initial concentration of DDT, initial concentration of Nitrogen as NH4NO3, inoculum size, and the concentration of glycerol as co-substrate. Another objective is to determine the best combination of the four factors in terms of the degree of DDT degradation. Last is the determination of the kinetics of DDT degradation.
The method to be used in the experiment is the use of a microbial biofilm. Biofilms will be developed in a fluidized bed reactor and Imhoff cones using modified cement particles as solid support. The inoculum will be obtained from a creek.
The biofilms will be acclimatized by gradual exposure to DDT and will be used to degrade DDT under varying conditions such as initial concentration of DDT, nitrogen concentration, inoculum size, and glycerol concentration. All degradation runs will be conducted in batch mode and the extent of degradation will be determined using GC-ECD. The time course of DDT degradation will be used to determine the kinetics of degradation.