Biochemistry - Effect of Temp. on Catalase Activity
Chemistry of Life Practical Investigation | Investigation of a Factor Affecting Enzyme Action | By: Jerry Li
02/03/2013
02/03/2013 | |
HL Biology
Term 1 Year 11
HL Biology
H
Contents 1 Design 2 1.1 Defining the problem 2 Research Question 2 Hypothesis 2 Background Information 2 Investigation Variables 3 1.2 Controlling Variables 4 Control Variables 4 Uncontrolled Variables 4 Control Experiment 4 1.3 Experimental Method 5 Materials and Equipment 5 Method 5 Practical Safety and Risk Assessment 6 Setup Diagram 6 2 Data collection and processing 7 2.1 Recording Raw Data 7 Sufficiency of Data 7 Results – Raw Data 7 Qualitative Observations: 8 2.2 Processing Data 9 Statistical Processing - Calculations 9 2.3 Presenting Processed Data 10 Results Table 10 3 Conclusion and Evaluation 11 3.1 Conclusion 11 Conclusion Statement 11 Conclusion Explanation 11 3.2 Evaluation Procedures: 11 Reliability 11 Errors/Limitations in Experimental procedure 11 Significance 12 3.3 Improving the Investigations 12 Suggestions for Improvements 12 Appendix 13 References 13 Journal 14
1 Design
1.1 Defining the problem
Research Question
This lab will be driven by the research question; do changes in temperature (from 0˚C, 7˚C, 21˚C, 37˚C, and 90˚C) have an effect on the activity of the enzyme catalase (found in beef liver) in the breakdown of hydrogen peroxide?
Hypothesis
If liver is placed in different temperatures of 0˚C, 7˚C, 21˚C, 37˚C, and 90˚C and hydrogen peroxide is added to each piece of liver, then the liver placed in 37˚C will have the largest reaction height. The liver in 90˚C will have the smallest reaction height, followed by the liver in 0˚C, then 7˚C, and then 21˚C.
Background Information
Pearson Baccalaureate: Standard Level
02/03/2013
02/03/2013 | |
HL Biology
Term 1 Year 11
HL Biology
H
Contents 1 Design 2 1.1 Defining the problem 2 Research Question 2 Hypothesis 2 Background Information 2 Investigation Variables 3 1.2 Controlling Variables 4 Control Variables 4 Uncontrolled Variables 4 Control Experiment 4 1.3 Experimental Method 5 Materials and Equipment 5 Method 5 Practical Safety and Risk Assessment 6 Setup Diagram 6 2 Data collection and processing 7 2.1 Recording Raw Data 7 Sufficiency of Data 7 Results – Raw Data 7 Qualitative Observations: 8 2.2 Processing Data 9 Statistical Processing - Calculations 9 2.3 Presenting Processed Data 10 Results Table 10 3 Conclusion and Evaluation 11 3.1 Conclusion 11 Conclusion Statement 11 Conclusion Explanation 11 3.2 Evaluation Procedures: 11 Reliability 11 Errors/Limitations in Experimental procedure 11 Significance 12 3.3 Improving the Investigations 12 Suggestions for Improvements 12 Appendix 13 References 13 Journal 14
1 Design
1.1 Defining the problem
Research Question
This lab will be driven by the research question; do changes in temperature (from 0˚C, 7˚C, 21˚C, 37˚C, and 90˚C) have an effect on the activity of the enzyme catalase (found in beef liver) in the breakdown of hydrogen peroxide?
Hypothesis
If liver is placed in different temperatures of 0˚C, 7˚C, 21˚C, 37˚C, and 90˚C and hydrogen peroxide is added to each piece of liver, then the liver placed in 37˚C will have the largest reaction height. The liver in 90˚C will have the smallest reaction height, followed by the liver in 0˚C, then 7˚C, and then 21˚C.
Background Information
Pearson Baccalaureate: Standard Level
References: Damon, Alan, Randy McGonegal, Patricia Tosto, and William Ward. Pearson Baccalaureate: Standard Level Biology for the IB Diploma (Pearson International Baccalaureate Diploma: International Editions). n/a: Imprint Unknown, 2008. Print. “Effect of Catalase on Hydrogen Peroxide.” http://www.sciencegeek.net/Biology/biopdfs/Lab_Catalase.pdf. (25 Jan. 2013). Journal