Biochemical Test Eneterobacteria

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Lab 6 Write Up: Biochemical

I. Purpose
The purpose of this lab was to become more familiar with Enterobacteriaceae Biochemical Tests.

II. Materials
*
* Phenol red dextrose broth
* Phenol red lactose broth
* Phenol red sucrose broth
* Casein agar plate
* Starch agar plate
* Litmus milk broth
* Gelatin deep
* Nitrate broth
* TSA slant
* Durham tubes
* Iodine
* 3% hydrogen peroxide
* Paminodimthylaniline oxalate
* Kovacs reagent
* Ferric Chloride
* Nitrate A
* Nitrate B
* Rubber gloves
* Sharpie
* Inoculating loop
* Inoculating needle
* Test tube rack
* Enterotube
* Distilled water
* Bunsen burner
* Bac Down
* Unknown Bacteria #

III. Methods

Phenol Red Dextrose, Lactose, and Sucrose with Durham tube:
The procedure for inoculation and interpreted results were used from Chess + Handout. A table was made and observations of growth, gas, and color for each bacterium were placed in the table. The broths were incubated at 35ºC for 24 hours.

Note: three broths of each type of sugar were used.
Casein Plate:
The procedure for inoculation and interpreted results were used from Chess + Handout. A table was made and observations of growth and Zone of hydrolysis for each bacterium were placed in the table. The plates were incubated at 35ºC for 24 hours

Note: Only 1 plate of Casein was used which was divide like a pie into three sections. Starch Plate:
The procedure for inoculation and interpreted results were used from Chess + Handout. . A table was made and observations of growth and iodine test results from each bacterium were placed in the table. The plates were incubated at 35ºC for 24 hours

Note:

Litmus Milk:
The procedure for inoculation and interpreted results were used from Chess + Handout. . Two tables were made which recorded bacteria’s growth, color, gas, and consistency. The broths were incubated at 35ºC for 24 hours.

Note:

Gelatin Deep:
The procedure for inoculation and interpreted results were used from Chess + Handout. A table was made and observations of growth and liquid for each bacterium were placed in the table. The deeps were incubated at 35ºC for 24 hours.

Note:

Nitrate Broth with Durham Tube:
The procedure for inoculation and interpreted results were used from Chess + Handout. A table was made and observations of growth, gas, and degree of reduction for each bacterium were placed in the table. The broths were incubated at 35ºC for 24 hours Note:

TSA Catalase Slants:
The procedure for inoculation and interpreted results were used from Chess + Handout. . A table was made and observations of growth and H2O2 presence for each bacterium were placed on the table. The slants were incubated at 35ºC for 24 hours.

Note:

Enterotube:
The procedure for inoculation and interpreted results were used from Chess + Handout. . The results were recorded on a pre-made Enterotube identification handout. The Enterotube was incubated at 35ºC for 24 hours.

Note:

API
The procedure for inoculation and interpreted results were used from Chess + Handout. The results were recorded on a pre-made API identification handout. The API strip was incubated at 35ºC for 24 hours.

Note:
Once the index value was found, the number was used to search for the unknown in the API Quick Index Booklet. . An oxidation test was performed to make sure the unknown would perform positive tests. IV. Results

Phenol Red Dextrose with Durham Tube
| | | |
Growth +/-| | | |
Gas +/-| | | |
Color| | | |
Phenol Red Sucrose with Durham Tube
| | | |
Growth +/-| | | |
Gas +/-| | | |
Color| | | |

Phenol Red Lactose with Durham Tube
| | | |
Growth +/-| | | |
Gas +/-| | | |
Color| | | |

Casein Plate
| | | |
Growth +/-| | | |
Zone of Hydrolysis +/-| | | |

Starch Plate
| | |...
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