Cellular respiration is the release of energy from organic compounds by metabolic chemical oxidation in the mitochondria in each cell. Cellular respiration involves a number of enzyme mediated reactions. There are three ways cellular respiration could be measured. The consumption of O2 Production of CO2 (how many moles of CO2 are produced in cellular respiration) and the release of energy during cellular respiration in this lab you will study cellular respiration in yeast cells. I will alter various factors that can affect the rate of cellular respiration. Comparison of different samples will allow you to determine what conditions are best for the yeast. I will vary the substrate provided to the yeast for use as fuel, I will vary pH, and will also investigate the effects of two inhibitors, Each inhibits cellular respiration by a different mechanism. I will measure the amount of CO(2) produced by the metabolizing cells to quantify the rate of cellular respiration. Hypothesis-As the time increases the amount of CO(2) produced will increases Materials—10 Test tubes, wax pencil, Respirometer, Yeast, Test Tube Rack, Water Method—First Wash the Test tubes with soap and water, Rinse and drain thoroughly needs to be clean. Then Measure the length Of the small Test tube and Divide by three and mark the spots on the small tubes at positions that are a 3rd of a length apart. Practice the assembly of the respirometer before u actually starts the experiment. After you do that u should fill the test tube to the top with liquid. Using your small finger or pencil insert the small test tube into the big one. Then quickly flip the tube or hold it at an angle all the liquid should stay inside the small test tube. Now do these again but this time add the shaken yeast to the top of the substrate. Then assemble the respirometers quickly. Respiration begins as soon as the yeast is added to the substrate this time is zero. Discussion- I wasn’t In Class for this lab but i...
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