Enzyme Lab Experiments
Problem: How can we demonstrate how enzymes work? What happens if we alter the environment of an enzyme? Materials:
G;lucose Test Strips
Potato Raw Liver
Dairy Lactose Tablet
If we change the environment via temperature the glow stick will Its intensity will change
If hydrogen peroxide is added to a certain food liver then It would bubble
If a lactaid enzyme to milk the
It would separate
Collect three glow sticks.
Boil water in 400 mL beaker add 1st glowstick for 30 minutes. 3.
Place second glowstick in freezer for 30 minutes
Leave third glow stick at room temperature for 30 minutes 5.
Observe and record findings.
Data Collected (A):
Place a glow stick in each environment below. Using a scale 1-3 1 being normal intensity 3 being brightest intensity
Room Temperature (control)
Collect sample of raw liver, potato, raw hamburg, lettuce and chalk. 2.
Place each sample in individual test tube in rack.
Observe samples prior to adding peroxide.
Add 1 mL of peroxide to each test tube
Observe and record findings
Data Collected (B):
Reaction after hydrogen peroxide is added. Using a scale of 0-5 0 Being no bubbles 5 being the most bubbles
Observation before adding hydrogen peroxide
Observation after adding hydrogen peroxide # 1 liver
Dark brown in color (expired meat)
# 2 potato
Inner slice with some skin
# 3 raw hamburg
Normal in color
# 4 lettuce
Fresh green lettuce
# 5 chalk
Yellow colored chalk
1. Enzyme Solution: Add one lactase tablet to 200 ml of water. Stir until the tablet is dissolved. 2.
Skim Milk: This solution contains lactose.
Add 5 grams of sugar to...
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