Beetroot Experiment

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What Effects Do Temperature
And Standard Solution
Have on a Beetroots
Cell Membrane.

Content:

* Aim.............................................................................................................

* Preliminary Work.......................................................................................

* Hypothesis.................................................................................................

* Risk Assessment.........................................................................................

* Method (Plan)............................................................................................

* Table of Results..........................................................................................

* Graphs........................................................................................................

* Analysis......................................................................................................

* Evaluation..................................................................................................

* Bibliography...............................................................................................

Aim:

The aim of the experiment that was carried out was to see, at what temperature and in which standard solution, does the amount of dye left in the boiling tube had the highest percentage transmission. Beetroot was used for the experiment because it was easier to see the dye after the practical was completed. Putting beetroot cylinders into different boiling tubes, which contained three different standard solutions of Ethanol, they were left for half an hour at a certain temperature to get the dye out. Once the time was up, the solutions was added to cuvettes and inserted into the colorimeter to test how much light was passed through the sample.

Preliminary Work:

Before the experiment was started, a preliminary experiment was needed to see if the actual experiment would give a good enough range of results. The preliminary was the same as the experiment but only did the extremes; at 0°C and at 70°C. The results were:

Temperature| Percentage Ethanol| Percentage Transmission| 0°C| 100%| 76%|
| 50%| 82%|
| 0%| 99%|
70°C| 100%| 2%|
| 50%| 7%|
| 0%| 5%|

From the results, it was easy to see that there is a clear range as at 0°C the highest was at 99% which indicated that hardly any betalain left the cell membrane and the sample in the cuvette was translucent. But then at the other end of the scale at 70°C, the lowest was only 2% transmission, meaning that most of the betalain in the membrane was released and left the cell and mixed with the Ethanol. So from the preliminary work, it was certain to say that there was a range from the highest and lowest temperatures and that for the experiment, there would be a suitable results table to create well presented graphs.

Hypothesis:

For the experiment it was believed that ethanol would have the effect on the cell membrane than water and that the more ethanol present, the lower the percentage transmission. So, pure ethanol should have the lower results, than the others and water should have the highest. The phospholipid bilayer which makes up the cell membrane can be described as a fluid structure in which proteins can move about dependent upon the fluidity of the lipids. In a fluid membrane one protein can change place with another or move to attach to another, thus facilitating function. Clearly the amount of fluidity of the membrane influences the function. Diminishing the fluidity decreases the ability of the proteins to move and interact, while enhancing the fluidity may lead to a disordered mess. Ethanol is shown to fluidize the bilayer and therefore decreasing the proteins ability to move. [1] The ethanol might also impair particular proteins or denature the structure as...
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