Bathroom Handle Bacteria
Working with Andie Shuck
Biology Survey Lab 101
Bacteria are among the first forms of life on earth. There is an average there are 40 million bacterial cells in a gram of soil and a million in just a milliliter of fresh water. Even humans have bacteria inside their organs and on their skin. Not all bacteria are harmful; some bacteria types are helpful and necessary for survival. Bacteria are generally a few micrometers in length and range in shapes of rods, spirals and spheres. In our lab experiment Andie and I took a swab and rubbed it all around the knob on the bathroom stall handle. We took samples from the boys and girls bathroom. Our hypotheses was that after 2 weeks of the bacteria growing in the agar, out of the two agers and the two sides, the boys in the incubator would have more bacteria then the girls in the incubator or room temperature and the boys at room temperature.
We started out experiment with collecting the two ager dishes. Then we divided both ager dishes in half; one side was for the men’s restroom and the other side was for the women’s. We labeled the sides and also labeled one ager dish to be stored at room temperature and the other to be incubated at 35 degrees Celsius. Once we had everything labeled. We had the boys go into them men’s restroom with a dry to swab the handle of the bathroom stall for us. While the boys did the men’s restroom, Andie and I swabbed the women’s stall knob. We made sure to swab the handle very well to insure the best results. Once we swabbed the knob, we rubbed it onto the women’s half of both the ager dishes. Once we were done with the women’s we repeated the process with the men’s side if the
ager, making sure we covered the same amount of area on the agar. Once they were swabbed and labled, we sealed them with tape and put one in the incubator and kept the other at room temperature to sit for two weeks.
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