ASSAY OF OIL-DEGRADING POTENTIAL OF FUNGI ISOLATES ON DIESEL, KEROSENE AND PETROL USING ENRICHMENT METHOD.
BOBOYE B., *OLUKUNLE O. F., ADETUYI F. C. AND ADEBIYI G. A
ABSTRACT
A study was carried out to assay for oil-degrading potential of fungi isolates on diesel, kerosene and petrol. Water samples were collected aseptically and analyzed microbiologically using standard techniques. The fungi isolated from the water samples were: Trichoderma viridae, Aspergillus niger, Aspergillus fumigatus and Stochyborys atra. The confirmatory test for oil utilization potential of the isolated fungi was carried out using the enrichment method, minimal salt broth (MSB). There were variations in the growth patterns of each of the fungal species with respect to the different oil (diesel, kerosene, petrol) used. This also signified that fungal species varied in their degradative abilities. The length of incubation for the fungi ranged from the 5th day to the 20th day.
The fungi with the highest hydrocarbon utilization potentials were: Aspergillus fumigatus and Aspergillus niger while stachyborys atra had the least hydrocarbon utilization potential. These hydrocarbon degraders are capable of utilizing oil polluted river and could therefore be employed in bioremediation process.
*Corresponding Author:
Institute of Microbial Biotechnology and Metagenomics, University of the Western Cape, South Africa
KEYWORDS: oil–degrading potential, microorganism, polluted land, hydrocarbon degraders, enrichment medium
INTRODUCTION
The huge demand for crude oil causes enormous quantities to be moved from production areas to different destinations where the oil will be used. The greatest environmental problem connected with crude oil exploration in Nigeria is oil spill both on-shore and off-shore (Okpokwasili, 1996). Crude oil, because of its characteristics, is one of the most significant pollutants in the environment as it is capable of causing serious damages to humans and... [continues]
BOBOYE B., *OLUKUNLE O. F., ADETUYI F. C. AND ADEBIYI G. A
ABSTRACT
A study was carried out to assay for oil-degrading potential of fungi isolates on diesel, kerosene and petrol. Water samples were collected aseptically and analyzed microbiologically using standard techniques. The fungi isolated from the water samples were: Trichoderma viridae, Aspergillus niger, Aspergillus fumigatus and Stochyborys atra. The confirmatory test for oil utilization potential of the isolated fungi was carried out using the enrichment method, minimal salt broth (MSB). There were variations in the growth patterns of each of the fungal species with respect to the different oil (diesel, kerosene, petrol) used. This also signified that fungal species varied in their degradative abilities. The length of incubation for the fungi ranged from the 5th day to the 20th day.
The fungi with the highest hydrocarbon utilization potentials were: Aspergillus fumigatus and Aspergillus niger while stachyborys atra had the least hydrocarbon utilization potential. These hydrocarbon degraders are capable of utilizing oil polluted river and could therefore be employed in bioremediation process.
*Corresponding Author:
Institute of Microbial Biotechnology and Metagenomics, University of the Western Cape, South Africa
KEYWORDS: oil–degrading potential, microorganism, polluted land, hydrocarbon degraders, enrichment medium
INTRODUCTION
The huge demand for crude oil causes enormous quantities to be moved from production areas to different destinations where the oil will be used. The greatest environmental problem connected with crude oil exploration in Nigeria is oil spill both on-shore and off-shore (Okpokwasili, 1996). Crude oil, because of its characteristics, is one of the most significant pollutants in the environment as it is capable of causing serious damages to humans and... [continues]
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(2010, 06). Assay of Oil-Degrading Potential of Fungi Isolates on Diesel, Kerosene and Petrol Using Enrichment Method.. StudyMode.com. Retrieved 06, 2010, from http://www.studymode.com/essays/Assay-Of-Oil-Degrading-Potential-Of-Fungi-338576.html
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"Assay of Oil-Degrading Potential of Fungi Isolates on Diesel, Kerosene and Petrol Using Enrichment Method." StudyMode.com. 06 2010. 06 2010 <http://www.studymode.com/essays/Assay-Of-Oil-Degrading-Potential-Of-Fungi-338576.html>.
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"Assay of Oil-Degrading Potential of Fungi Isolates on Diesel, Kerosene and Petrol Using Enrichment Method.." StudyMode.com. 06, 2010. Accessed 06, 2010. http://www.studymode.com/essays/Assay-Of-Oil-Degrading-Potential-Of-Fungi-338576.html.
