REG NUMBER : H1111895N
LECTURER : MR KUTYAURIPO
COURSE : MICROBIOLOGY
PRACTICAL : 2
TITLE : STERILISATION BY AUTOCLAVING & ASEPTIC TECHNIQUES IN POURING OF
DUE DATE :10/27/2011
Experiments were carried out which involved autoclaving and aseptic techniques in pouring of plates .Experiment 1 was carried out to show that auto claving (steam heating under pressure ) destroys microorganisms .Nutrient agar was prepared and autoclaved for 15 minutes , after which it was poured into six test tubes with soil samples added to the tubes. The test tubes were incubated for 72 hours . In test tube 1,2,3 which had caps no growth occurred due to the absence of microbes due to the assumption that the Broth did not support growth of the microbes in the soil sample or that the the soil sample was sterile. Test tubes 4,5,6 which had no caps ,growth was noted as air was not hindered from entering and this shows that microbes do exist in the air. In experiment 2, nutrient agar was prepared autoclaved and poured into eight petri dishes , observing aseptic techniques .Two petri dishes were inverted and incubated for 72 hours at 30 degrees. No growth was observed and this satisfied the objective of the practical which was, observing aseptic pouring techniques of the agar into petridishes INTRODUCTION
Sterile equipment contains no living organisms that interfere with the laboratory analysis of a specimen (Anderson ,2004). Due to the need to successfully grow pure cultures for identification and analysis it is essential to clean and sterilise all laboratory equipment before use. An autoclave operates on the same...