Ap Biology Lab 5

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AP BIOLOGY Lab 5 Cellular Respiration

Objective: To calculate the rate of CR from the data. To then relate gas production to respiration rate. Then test the rate of CR in germinating versus non-germinating seeds in a controlled experiment and then test the effect of temperature on the rate of CR in the germinating versus non-germinated seeds in a controlled experiment.


1.The CR is higher in the germinating peas in the cold water rather than in the beads or non-germinating peas. The cold water probably slowed the process of CR in both germinating and non-germinating peas. 2.The conditions that must remain constant are: the water baths, the same amount of KOH, the same amount of time for equilibration, the same time measurements, and using glass beads as the control. These must remain the same to ensure correct and reliable readings. If any of these were changed, results would dramatically change. 3.See Graph 5.1.

4.Over the time allotted, there was a slow increase of O2 consumed over the time span. 5.See Table 5.2.
6.The glass beads cannot produce gas, so it served as a control, due to no change in the volume of gas. 7.Germinating seeds have an increased metabolic rate, which in turn needs more oxygen. The non-germinating peas are smaller and barely alive, so they only need a little bit of oxygen to live. 8.See Graph 5.2. I think the graph will rise to a max peak of 40 C, where it’ll consume the most, then begin to decline as the heat increases past 50 C. 9.It was used to absorb excess CO2 and to make it precipitate at the bottom of the vial, so as to avoid any disruptions with the readings. 10.The stopper was simply put to keep what is in is in and what is out is out, i.e. gas in, water out. 11.I think respiration would be much higher in the mammal because they are warm blooded and in turn, endothermic. 12.Since the entire contraption was under water, the oxygen originally inside the vial was consumed by the peas, and the CO2...
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