牋牋牋牋The substance being acted on by the enzyme is known as the substrate, and in this case is hydrogen peroxide. The enzyme changes these substrates into products, in this case water and O2 gas. The purpose of this lab is to observe and measure the rates of conversion of hydrogen peroxide to water and oxygen by the enzyme catalase over different periods of time.
牋牋牋牋To measure the rate of the reaction, we measure the rate of disappearance of the substrate of over time. Potassium permanganate (KMn04) is used to indicate the presence of any remaining H2O2 in the solution after the reaction. The amount of remaining substrate is an indication of how often the conversion reaction took place.
牋牋牋牋After allowing an enzyme to act on a substrate solution for different periods of time and measuring the amount of remaining substrate, a rate of reaction can be calculated. And that is what the experiment boils down to, figuring out the rate of an enzyme catalyzed reaction. I predict that the rate for reaction will remain fairly constant over time.
牋牋牋牋Materials- 路 Burette 牋牋牋牋路 2% KmnO4 牋牋牋牋 牋牋牋牋路 1M H2SO4 (Sulfuric acid) 牋牋牋牋路 H2O (Water) 牋牋牋牋路 Potato 牋牋牋牋路 1.5% H2O2 (Hydrogen Peroxide) 牋牋牋牋路 Catalase 牋牋牋牋路 1 ml syringe 牋牋牋牋 牋牋牋牋路 10 ml syringe 牋牋牋牋路 Stopwatch 牋牋牋牋路 Six 50 ml beakers 牋牋牋牋路 2 more beakers (any fairly large size) to store acid and hydrogen peroxide 牋牋牋牋Procedure- Part A: Demonstration of Presence of Catalase in Living Tissue.
Note: Demonstration done by...