Anti Oxidant

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  • Topic: Antioxidant, Vitamin C, Ascorbic acid
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  • Published : March 24, 2013
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IN-VITRO ANTI-OXIDANT ACTIVITY OF THE PETROLEUM ETHER EXTRACT OF YUCCA GLORIOSA L. Rajendar Rao.V*, B.N.V. Bhargavi, Soujanya.G
Address for Correspondence: Rajender Rao.V
Sri Balaji College Of Pharmacy, Karimnagar, A.P, India.

ABSTRACT
The present study was undertaken to evaluate the in vitro antioxidant activities of whole plant of petroleum ether extract of Yucca gloriosa L. The plant extract was tested for DPPH (2, 2-diphenyl, 2-picryl hydrazyl) radical scavenging, and reducing power assays. The results show that the plant extracts of Yucca gloriosa L. possess antioxidant activity when compared with standard shows the same. Keywords: Yucca gloriosa L.,DPPH, Reducing Power Assay.

INTRODUCTION
Antioxidants are substances that may protect cells from the damage caused by unstable molecules known as free radicals. Antioxidants interact with and stabilize free radicals and may prevent some of the damage free radicals might otherwise cause. Antioxidant acts as cell protectors. Oxygen, an essential element for life, can create damaging by by-products during normal cellular metabolism. Antioxidants counteract these cellular by-products during normal cellular metabolism. Antioxidants counteract these cellular by-products called free radicals, and blind with them before they can cause damage. If left unchecked, free radicals may cause heart damage, cancer, cataracts, and a weak immunes system. Antioxidants come in a variety of forms include vitamin C, vitamin E, Carotenoids [1,2]. Yucca gloriosa L. commonly known as Spanish Dagger and Family- Agavaceae. It is a stermsless or rising of stature of small trees and trunk short. This palnt leaves are 2-3 feet long, 2 in wide, long pointed, often tooth margin, mostly in rosettes at surface of ground or ends of trunk. Flowers has many cup or saucer shaped, hanging, greenish white to reddish, fragrant, born mostly in erect panicles that usually overtop the leaves. The whole plant of Yucca gloriosa L. is used for ulcer, asthma and bronchitis [3]. From the source of literature documentation and relevant traditional approaches on plant drugs, the present investigation was carried out to investigate the invitro anti-oxidant activity of the Petroleum Ether Extract of Yucca gloriosa L. whole plant (PYG) is being reported here.

MATERIALS AND METHODS
Plant material
The whole plant of Yucca gloriosa L. was collected from Tirumala hills, Tirupati, Andhra Pradesh. India. It was identified and authenticated by Prof. Madhava Chetty, K., Taxonomist, S.V. University, Tirupati, Andhra Pradesh, India. A voucher specimen has been kept in our laboratory for future reference.

Preparation of plant extract
The collected whole plant was dried at room temperature, pulverized by a mechanical grinder, sieved through 40mesh. About 100g of powdered materials were extracted with petroleum ether (60°-80°C) using soxhlet apparatus. The extraction was carried out until the extractive becomes colourless. The extracts is then concentrated and dried under reduced pressure. The solvent free semisolid mass thus obtained is dissolved in tween 80 and used for the experiment. The percentage yield of prepared extract was around 10.5%w/w.

ANTIOXIDANT ACTIVITY
DPPH method
This is the most widely reported method for screening of anti oxidant activity of many plant drugs. DPPH assay method is based on the reduction of methanol solution of coloured free radical DPPH by free radical scavenger. The procedure involves measurement of decrease in absorbance of DPPH at its absorption maxima of 516 nm, which is proportional to concentration of free radical scavenger added to DPPH reagent solution. The activity is expressed as effective concentration EC50. The super oxide radical can also be detected by oxidation of hydroxylamine, yielding nitrite which is measured colorimetric reaction. Reducing power method

This method is based on the principle of increase in the absorbance of the reaction mixture. Increase...
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