Angeline Veth
Angeline Veth
Period 1
A.P Biology
Introduction:
How can the process of transformation effect bacteria cells?
-Our independent variable were Plasmids, the three genes on our plasmid are small and circular, self-replicating chromosome in bacteria.
-Our dependent variable was florescent, ampicillin resistant bacteria.
-The control was the plates were the one without plasmids which was LB/AMP/+PGLO, LB/AMP/-PGLO, LB/-PGLO. These threes represented nutrients and ampicillin, plasmids and arabinose.
-The purpose to bacterial transformation is to insert DNA into bacteria to change it’s traits.
Materials: Quantity:
E. coli starter plate 1
Poured agar plates 4
Transformation solution 1
LB nutrient broth 1
Inoculation loops 5
Pipets 1
Foam micro centrifuge tube 1
Container of ice cubes 1
Marking pen 1
Copy of procedures 1
Mirocentrifuge tube 2
Rehydrated Pglo Plasmid 1 vial
42C water bath and thermometer 1
UV light 1
37 C incubator 1
Procedures:
Label one closed micro test tube +PGLO and another –PGLO. Label both test tubes with your groups name. Place them in the foam tube rack.
Open the tubes and, using a sterile transfer pipet, transfer 250 ul of transformation solution (CaCL2) into each tube.
Place the tubes in the ice.
Use a sterile loop to pick up 2-4 large colonies of bacteria from your starter plate. Select starter colonies that are “fat” (ie:1-2 mm in diameter). It is important to take individual colonies (not a swab of bacteria from the dense portion of the
Period 1
A.P Biology
Introduction:
How can the process of transformation effect bacteria cells?
-Our independent variable were Plasmids, the three genes on our plasmid are small and circular, self-replicating chromosome in bacteria.
-Our dependent variable was florescent, ampicillin resistant bacteria.
-The control was the plates were the one without plasmids which was LB/AMP/+PGLO, LB/AMP/-PGLO, LB/-PGLO. These threes represented nutrients and ampicillin, plasmids and arabinose.
-The purpose to bacterial transformation is to insert DNA into bacteria to change it’s traits.
Materials: Quantity:
E. coli starter plate 1
Poured agar plates 4
Transformation solution 1
LB nutrient broth 1
Inoculation loops 5
Pipets 1
Foam micro centrifuge tube 1
Container of ice cubes 1
Marking pen 1
Copy of procedures 1
Mirocentrifuge tube 2
Rehydrated Pglo Plasmid 1 vial
42C water bath and thermometer 1
UV light 1
37 C incubator 1
Procedures:
Label one closed micro test tube +PGLO and another –PGLO. Label both test tubes with your groups name. Place them in the foam tube rack.
Open the tubes and, using a sterile transfer pipet, transfer 250 ul of transformation solution (CaCL2) into each tube.
Place the tubes in the ice.
Use a sterile loop to pick up 2-4 large colonies of bacteria from your starter plate. Select starter colonies that are “fat” (ie:1-2 mm in diameter). It is important to take individual colonies (not a swab of bacteria from the dense portion of the