Section: AP 2 – 131 – Fall 2010
Laboratory Report: Exercise 1: The Endocrine System
Purpose: What is the purpose of this exercise? Is there any safety concerns associated with this exercise? If so, list what they are and what precautions should be taken.
1. Identify the major endocrine glands and other organs containing endocrine cells on models and diagrams (glands listed below). 2. Identify the major endocrine glands in the cat (glands listed below). 3. Identify the histological features (listed below) of the major endocrine glands on microscope slides. 4. Identify the endocrine organ from which a slide is prepared when viewing the slide with a microscope.
With any lab or scientific experiment there is always risk involved. It is imperative for one to be observant of their surroundings while conducting the lab to ensure safety at all times. This lab requires the use of a Microscope and Glass Slides. The glass of the slides is thin and should be handled gingerly to prevent cracking or breaking, which may result in injury. Additionally, it is imperative to use the Microscope correctly to prevent breaking slides or dropping the scope.
Activity 1: The Pituitary Gland
Observations: Please describe in detail the structures and features you observed on the pituitary gland slide.
The pituitary gland was observed to be in 2 distinct parts and separated by a middle ridge (little ravine). I observed the gland to be composed of two tissue types, an adenohypophysis (anterior lobe) derived from buccal ectoderm and a neurohypophysis (posterior lobe) also called pars nervosa derived from neural ectoderm. The adenohypophysis contains mainly cords of glandular epithelium surrounded by blood vessels and some C.T. The major anterior portion of this lobe is called pars distalis. A smaller portion, pars intermedia, lies between the pars distalis and the posterior lobe neurohypophysis. The posterior lobe contains mainly nerve processes and endings, (the terminal portion of the hypothalamo-hypophyseal tract) along with glial cells, blood vessels and some C.T. Reddish brown acidophil and dark blue basophile are clearly discernible and have numerous granules.
Data Table 1: Do some research and fill in the data table below:
|Hormone |Functions | |Adrenocorticotropic hormone (ACTH) |Its principal function is stimulating the adrenal gland cortex| | |to secrete a group of steroid hormones called glucocorticoids.| |Antidiuretic hormone (ADH) |The single most important effect of antidiuretic hormone is to| | |conserve body water by reducing the loss of water in urine. | |Follicle stimulating hormone (FSH) |In women, FSH helps control the menstrual cycle and the | | |production of eggs by the ovaries. The amount of FSH varies | | |throughout a woman's menstrual cycle and is highest just | | |before she releases an egg (ovulates). | | |In men, FSH helps control the production of sperm. The amount | | |of FSH in men normally remains constant. | |Growth hormone (GH) |HGH is the "master hormone" controlling many organs and body | |...