Journal of the Chinese Chemical Society, 2011, 58, 236-240
Analytical Characterization of Fatty Acids Composition of Datura alba Seed Oil by Gas Chromatography Mass Spectrometry
Muhammad Nasimullah Qureshi,* Muhammad Siddique, Inayat-ur-Rahman and Farina Kanwal
Medicinal Botanic Centre, PCSIR Laboratories Complex Peshawar, Pakistan
Received July 9, 2010; Accepted November 23, 2010; Published Online December 14, 2010
Methyl ester derivatives of fatty acids were analyzed for the determination of the constituents of Datura seed oil. Gas chromatography coupled to mass spectrometer was used for these analyses. Results delivered that there were saturated as well as unsaturated fatty acids in Datura alba seed oil. Total of 15 different fatty acid components were identified and quantified. Methyl linoleate was found in highest concentration (16.22%) among the identified analytes of interest. In addition methyl esters of Palmitic acid (6.59%), Oleic acid (5.41%) and Stearic acid (1.35%) were found. Concentrations of rest of the detected fatty acids were less than 1%. From the literature it appears that no such work has been performed for the determination of fatty acids in Datura alba seed oil. Keywords: Datura alba; Fatty acids; Methylation; GC-MS.
1. INTRODUCTION Datura alba (Family; Solanaccae) grows in warmer parts of the world particularly in south and south east Asia including Pakistan, India and Sri Lanka. This annual herb is bushy, smooth, fetid, 0.5 to 1.2 m in height also attaining 6 feet or more in rich soils. The leaves are 18 cm long and the flowers are white in colour.1,2 Besides its familiarity for toxicity and poisoning,3 it has uses for a number of diseases like asthma, muscle spasm, whooping cough, skin ulcer, hemorrhoids, anti-rheumatic etc. Its oil based preparation is used for healing of all types of wounds in Ayurveda and Siddha practice of medicine since long ago.4 Datura species produces a number of small seeds encapsulated in an... [continues]
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