For something to be in Hardy-Weinberg equilibrium five conditions must be met: random mating, no mutations, no migration, infinite size/population and no sexual/natural selection. Since the equilibrium is met in this experiment, it suggests all 5 conditions apply to the population. Mating is random and no natural selection occurs as the Alu insertion genotypes have no phenotypes nor give any particular advantage to the individual (Lewis, 2007). The population is considered infinite because there are enough individuals once the class data is combined. Mutations occur randomly, but the fact that it is in Hardy-Weinberg equilibrium means that if mutations do occur the rate at which it does is so minimal that it has little to no affect. Migration most likely occurred among the parental generation, but because the equilibrium was kept, it suggests that no one allele were disproportionally added or removed. Thus, with the five conditions met, Hardy-Weinberg equilibrium is sustained on all alleles.
Some individuals’ PCR product was undetectable when examined under a transilluminator. Multiple factors can attribute to this. One possibility is that an adequate amount of centrifugation and boiling did not occur to lyse the nuclear membrane, stopping the primer from attaching to the DNA, resulting in no PCR product. Another factor is that mutations or contaminations could have prevented the primer from attaching, meaning no amplification to detect the product. Lastly, deficient amounts of Chelex 100 and/or master mix could have been used which are used to stop PCR inhibitors and provide reagents necessary for amplification, which would lead to no band (Scortichini et al, 1998). Appendix A
Table 1: Observed frequencies of three genotypes of tPA-25 insertion. Observed Genotype100bp/100bp
(tPA-25 absent/tPA-25 absent)400bp/100bp
(tPA-25 present/tPA-25 absent)400bp/400bp
(tPA-25 present/tPA-25 present)
Null Hypothesis: The alleles that encode for tPA-25 insertion are in Hardy-Weinberg equilibrium, therefore there is no significant difference between observed and expected values. Alternate Hypothesis: The alleles that encode for tPA-25 insertion are not in Hardy-Weinberg equilibrium, therefore there is a significant difference between observed and expected values. Table 2: Chi-squared test of observed and expected values for the three possible TPA-25 genotypes. GenotypeObserved FrequencyExpected Frequency(O-E)2/E
(tPA-25 absent/tPA-25 absent)27320.7813
(tPA-25 present/tPA-25 absent)61521.5577
(tPA-25 present/tPA-25 present)17210.7619
Chi square value = 3.1009
p = 0.0783
df = 1
At a critical value of 3.84 and significance level of 0.05, the...