Absorption Spectroscopy to Determine the Molar Concentration of a Solution

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Absorption Spectroscopy Lab


The concentration of the red dye in the Gatorade sample was 6.12x10^-5 g/mol. Also, from the data, we can detect that an average human being weight 60 kg, would have to consume 11,849,510 g of this sample to reach the LD50. Using Absorption Spectroscopy to determine the molar concentration of a solution is affective when there is a stock solution with a known concentration, which in this case is Red dye #40.


There are some dangers posed by the chemicals found in the food we eat and in drinks. Measuring the amounts of these dangerous chemicals is very important when determining their safety. Food safety is a continuing public concern in the United States, and the food nearly everyone eats has been processed in some form. During processing, chemicals are added to improve food safety by retarding spoilage and preventing the growth of unwanted organism. Some of the chemicals may be deceiving and this is our primary concern to be aware of. Chemicals that are dangerous in large amounts can be quite beneficial in small amounts. Measuring the amounts of chemicals present in food and drugs is really important, and the common way to measure this done by using absorption spectroscopy.

A most common chemical in the food we eat is food dye. The FDA is in charge of assessing and certifying the safety of chemicals that are added to food and used in drugs everyday, and to this day only nine food dye chemicals are safe for use. The dyes are made up of small organic molecules that absorb light in the visible region.

The purpose of this experiment is to identify the amount of dye present in a food product and evaluate its safety. The objective is to prepare a series of standards from a known solution using a serial or parallel dilution, prepare an unknown sample, measure absorbance of all samples, determine molar absorptivity of a food dye, and determine percent composition of the food dye in a food sample by...
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