Here are your goals for this lesson:
• To conduct tests for glucose, starch, lipids and proteins • Use these test results to identify the biological molecules found in food
Student Name: Brittany Gerdes
Date Experiment done:12-20-12
Date Report Completed: 12-20-12
Name of Grader:
Hypothesis: A prediction of what you think the results of the project will be. Write your hypothesis before you begin the experiment and, if the experiment has more than one part, you will need a hypothesis for each section. Remember to create an ‘if-then’ statement. (Example: If students get adequate rest, then grades will improve). Refer to the first Project of the semester or to the Student References Section if you need additional information (1-2 sentences)
Procedure: In 1st person past tense, write a summary of what you actually did in performing the experiment. Be sure to include any modifications. You should include enough detail so that someone could reproduce the experiment based on what you have written
Data: Describe all observations or changes. List all measurements and include proper labels.
Calculations/Interpretations: Show all math performed (label the calculation or give the formula, show your setup, and give the result), include proper labels, and/or answer any questions listed below.
Conclusion: Begin by stating whether the hypothesis was true or false. Use data and calculations to support your answer. Consider the following questions as you write your conclusion: Why or what happened to result in the outcome you observed? Did you learn anything new? If not, what previously concepts did this lab reinforce? Is there anything you would or could do differently that would improve the experiment? Do you have any other comments/observations you would like to share about this lab? (*Note: Not all questions pertain to each lab.) Your conclusion should be approximately one paragraph in length.
The experiment will test the presence of biological molecules found in food. If glucose molecules are found, the solution color will change to green to yellow to orange depending on the quantity of sugars present in the solution.
Experiment 1: Testing for Glucose (Simple Sugar)
In the experiment I used two test tubes, one with 3 mL of glucose solution and the other with 3 mL of water. Then I added 3 mL of Benedict’s Solution to each tube. The color change was noted then I heated the tubes in the constant temp. bath to 100C. Again, the color of the solution was noted.
I used two additional clean test tubes and 3mL of Fructose solution was added to one and 3mL of Sucrose was added to the other. Then I added 3 mL of Benedicts Solution to each tube and heated to 100C. The change in color would indicate whether the test was positive or negative for the presence of a reducing sugar.
When the glucose solution and the Benedicts solution were combined, there was not a color change which indicated a negative result for glucose. But when the Fructose and the Benedicts solution were combined and heated, the color change to orange indicated the presence of glucose. The Sucrose and Benedicts solution did not test positive in my experiment.
Complete the following table:
|Solution |Color | | | | |Benedict’s solution alone |Blue | |Glucose solution alone |Clear | |Benedict’s solution and Glucose solution |Blue...