Unknown Lab Report

Only available on StudyMode
  • Topic: Enterobacteriaceae, Enterobacteria, Microbiology
  • Pages : 6 (1055 words )
  • Download(s) : 1375
  • Published : March 24, 2013
Open Document
Text Preview
Unknown Lab Report #1

Unknown #1
April 25, 2012
Microbiology
Spring 2012
MCB2010C

Unknown #1
Introduction
Identity of a microorganism has proven to be very significant. Doing so can help identify diseases and created treatment and cures for such diseases. As a result, various laboratory tests were performed to an unknown microbe (Unknown #1) found in the water of a nearby pond. By identify the microbe, the safety of the water will be known to those around it. Materials and Methods

Unknown #1 was found in a nearby pond that was created by an earthquake. Some of the various methods introduced and practiced in class were applied in identifying the microbe. Procedures were followed as stated in the index of the Virtual Unknown Software. First the shape and color of the microbe was noted. This is done to know the morphology of the microbe. After concluding that the microbe was Gram negative rods, it was inoculated into a previously prepared test tube. Then various tests were performed to isolate and identify the unknown microbe. Table 1 shows the tests, purpose, reagents and results of the various tests performed. Each test was performed according to the index of the Virtual Unknown software and the following tests were performed: 1) Adonitol Fermentation

2) Cellobiose Fermentation
3) Maltose Fermentation
4) Lactose Fermentation
5) Raffinose Fermentation
6) Sorbitol Fermentation
7) Sucrose Fermentation
8) Melibiose Fermentation
9) Indole Production
Results
Table 1: Biochemical Tests Results
Test| Purpose| Reagents| Observations| Results|
Adonitol Fermentation| To determine if the microbe can ferment the carbohydrate (sugar) adonitol as a carbon source.| None| Turned pink| Negative adonitol test| Cellobiose Fermentation| To determine if the microbe can ferment the carbohydrate (sugar) cellobiose as a carbon source.| None| Turned Yellow| Positive cellobiose fermenter| Maltose Fermentation| To determine if the microbe can ferment the carbohydrate (sugar) maltose as a carbon source.| None| Turned Yellow| Positive maltose fermenter| Lactose Fermentation| To determine if the microbe can ferment the carbohydrate (sugar) lactose as a carbon source.| None| Turned Pink| Negative lactose fermenter| Raffinose Fermentation| To determine whether the microbe can use sugar raffinose for carbon and energy.| None| Turned pink| Negative raffinose fermenter| Sorbitol Fermentation| To determine if the microbe can ferment the carbohydrate sorbitol as a carbon source.| None| Turned yellow| Positive sorbitol fermentation| Sucrose Fermentation| To determine if the microbe can ferment the carbohydrate sucrose as a carbon source| None| Turned Pink| Negative sucrose fermenter| Melibiose Fermentation| To determine if the microbe can ferment the carbohydrate (sugar) melibiose as a carbon.| None| Turned Pink| Negative melibiose fermenter| Indole Production| To determine whether the microbe can produce indole from the amino acid tryptophan.| Add five to ten drops of Kovac’s reagent to the test tube. The reagent does not mix with water and forms a thin layer above the broth. The reagent reacts with idole to produce a cherry red ring.| Red ring at the top of broth| Positive indole test|

Flowchart
Unknown#1

Gram Stain

Gram Negative Rod

Adonitol Test (-)

Positive Negative
Klebsiella ornithinolyticaCitrobacter koseri
Klebsiella oxytocaEnterobacteraergenes
Klebsiella oxytocaEscherichia fergusonii
Klebieslla pneumoniae sspKlebsiella ornithinolycia
pneumoniaKlebsiella pneumonia
Klebsiella oxytocassp ozaenae
Klebsiella terrigenaKlebsiella pneumonia ssp
Leclercia adecarboxlatarhinosclermatis
Klebsiella terrigena
Leclercia adecarboxylata
Mollerlla wisconsinisis
Providencia alcalifaciens
Providencia rettgeri
Serratia fonticola...
tracking img