Standard Curve Preparation

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Clinical Chemistry
Manlangit, Joyce Ann U.

EXPERIMENT
Title: Standard Curve Preparation
Objectives:
1. Properly set up a standard curve
2. Using the prepared curve, determine the concentrations for control and patient specimens. Materials and Reagents:
2ml 1M Sulfuric Acid
2ml of 0.1M Potassium Dichromate
Distilled Water
Pipettes
Volumetric Flask
Test Tubes
Procedure:
1. Prepare 1 ml glucose solution and place in test tubes
2. Add 2ml of 1M Sulfuric Acid solution
3. Add 2ml of 0.1M Potassium Dichromate solution
4. Start timing until the color disappear

Results and Discussion:

The fastest concentration of glucose to react with oxidation-reduction is the 1.0% concentration while the slowest to react is 0.1% concentration. Conclusion:
I have therefore conclude that the higher the concentration of glucose, the faster disappearance of colour indicating the oxidation-reduction reaction. Questions:
1. Identify the possible source of error in the activity. a. Contamination that will alter the reaction
b. Improper use of pipette that will cause inaccurate measurement c. Inaccurate timing that will lead to wrong erroneous plotting of result 2. How can the activity be more efficient?
a. Observe the highest concentration if you have limited time because it is the fastest one to react, thus, giving you an extra for the other concentration especially the lowest one. b. If using one pipette, always wash it after transferring the other concentration to test tube. Using one pipette for transferring all the concentration to test tube will cause contamination, thus, giving you inaccurate result.
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