Questionnaire on Nucleic Acid

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  • Topic: DNA, Polymerase chain reaction, DNA sequencing
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  • Published : February 24, 2013
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Assignment 2: Answer Sheet (LSM 1401 Semester 2 AY2011/12)

Nucleic Acid Virtual Laboratories

|Name | | |Matriculation number | | |A. DNA Extraction Virtual Lab [2 marks] | |Question 1 [1.0 mark] | |Isolate nucleated cells into eppendorf tube (correct starting step). | |Add 500 ul 10% SDS and 55 ul proteinase K (10 mg/ml stock).  | |Incubate at 37ºC with gentle mixing or rocking.  | |Add 1.4 ml saturated NaCl solution (approximately 6M).  | |Spin eppendorf tubes at 10000 rpm in a centrifuge for 15 minutes.  | |Shake the tube vigorously for 15 seconds to allow protein to precipitate.  | |Transfer the supernatant to another eppendorf tube, leaving behind the precipitated protein pellet | |Add exactly two volumes of 100% isopropanol at room temperature.  | |Spin eppendorf tubes at 2500 rpm in a centrifuge for 15 minutes.  | |Invert the tube several times until the DNA precipitate is visible. ...
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