Principles of Gene Manipulation
Contents and supplementary information for: Principles of Gene Manipulation
Chapter 1 Chapter 2 Chapter 3 Chapter 4 Chapter 5 Chapter 6 Chapter 7 Chapter 8 Chapter 9 Chapter 10 Gene manipulation: an all-embracing technique Basic techniques - (POGC02.pdf, 1,560KB) Cutting and joining DNA molecules Basic biology of plasmid and phage vectors Cosmids, phasmids and other advanced vectors Cloning strategies Additional updated information on Cloning strategies Sequencing and mutagenesis Cloning in bacteria other than E. coli Cloning in Saccharomyces cerevisiae and other fungi Gene transfer to animal cells Additional updated information on Gene transfer to animal cells Chapter 11 Genetic manipulation of animals Additional updated information on Genetic manipulation of animals Chapter 12 Chapter 13 Gene transfer to plants Additional updated information on Gene transfer to plants Advances in transgenic technology Additional updated information on Advances in transgenic technology Chapter 14 or (POGC13.pdf - size: 353KB)
Applications of recombinant DNA tecnology
Overview / Supplememtary Material / Related Titles / Related Websites / Ordering Information / Examination Copies / MCQ 's / New Edition
CH A P T E R 1
Gene manipulation: an all-embracing technique
Introduction
Occasionally technical developments in science occur that enable leaps forward in our knowledge and increase the potential for innovation. Molecular biology and biomedical research experienced such a revolutionary change in the mid-70s with the development of gene manipulation. Although the initial experiments generated much excitement, it is unlikely that any of the early workers in the field could have predicted the breadth of applications to which the technique has been put. Nor could they have envisaged that the methods they developed would spawn an entire industry comprising several hundred companies, of varying sizes, in the USA alone. The term gene manipulation can be applied to a variety
Chapter 1 Chapter 2 Chapter 3 Chapter 4 Chapter 5 Chapter 6 Chapter 7 Chapter 8 Chapter 9 Chapter 10 Gene manipulation: an all-embracing technique Basic techniques - (POGC02.pdf, 1,560KB) Cutting and joining DNA molecules Basic biology of plasmid and phage vectors Cosmids, phasmids and other advanced vectors Cloning strategies Additional updated information on Cloning strategies Sequencing and mutagenesis Cloning in bacteria other than E. coli Cloning in Saccharomyces cerevisiae and other fungi Gene transfer to animal cells Additional updated information on Gene transfer to animal cells Chapter 11 Genetic manipulation of animals Additional updated information on Genetic manipulation of animals Chapter 12 Chapter 13 Gene transfer to plants Additional updated information on Gene transfer to plants Advances in transgenic technology Additional updated information on Advances in transgenic technology Chapter 14 or (POGC13.pdf - size: 353KB)
Applications of recombinant DNA tecnology
Overview / Supplememtary Material / Related Titles / Related Websites / Ordering Information / Examination Copies / MCQ 's / New Edition
CH A P T E R 1
Gene manipulation: an all-embracing technique
Introduction
Occasionally technical developments in science occur that enable leaps forward in our knowledge and increase the potential for innovation. Molecular biology and biomedical research experienced such a revolutionary change in the mid-70s with the development of gene manipulation. Although the initial experiments generated much excitement, it is unlikely that any of the early workers in the field could have predicted the breadth of applications to which the technique has been put. Nor could they have envisaged that the methods they developed would spawn an entire industry comprising several hundred companies, of varying sizes, in the USA alone. The term gene manipulation can be applied to a variety
References: Svab et al. 1990a Hille et al. 1986 Eichholtz et al. 1987 Van den Elzen et al. 1985 Pridmore 1987 Hayford et al. 1988 De Block et al. 1987 Haughn et al. 1988 Guerineau & Mullineaux 1989 Shah et al. 1986 continued CHAPTER 12 Bsp HI (3031) pSa-ORI Bgl II (536) Hpa I (568) LB nptI pGreenII 0000 3304 bp lacZ Asp 7181 (783) Apa I (793) Xho I (798) Sal I (804) Cla I (814) Eco RV (827) Eco RI (831) Pst I (841) Sma I (845) Bam HI (849) Spe I (855) Xba I (861) Not I (868) Sac II (880) Sac I (889) RB Bsp HI (2070) ColEI ori Stu I (1292) Bgl II (1345)