Microbiology Study Guide Chpt 1-6

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Microbiology Study Guide Chapters 1-6
Chapter 1 Main Themes of Microbiology
Most are not Pathogens
Prokaryotic-no nucleus or organelles
Very simplistic—like bacteria
Eukaryotic-has nucleus and organelles like mitochondria
Includes fungus, and animals
Acellular-not even a complete cell
Includes viruses and prions like those that cause Mad Cow disease
Six main types of microorganisms
Bacterium (prokaryotic)
Fungus (eukaryotic)
Algae (eukaryotic)
Virus (acellular)
Protozoan (eukaryotic)
Helminth (eukaryotic)
Most common infectious diseases worldwide
Respiratory #1
Diarrheal #3
Antonie van Leeuwenhoek
Fashioned together a primitive microscope—he was the first to observe microorganisms
Scientific method-an experimental system that answered questions objectively
Hypothesis- tentative explanation to account for what has been observed or measured; predicts specific explanations that may or may not be borne out by testing; best written as a statement
Experimentation-outlines particular events predicted by hypothesis, then sets up experiments to test for the events
Results-support or refute hypothesis (you never prove anything), some part of hypothesis may be discarded or modified to fit results
Conclusion or theory-repeated investigation of results
History of Microbiology
Spores and Sterilization
Some microbes in dust and air were resistant to high heat—later identified as spores
The term “sterile” was introduced which meant completely eliminating all life forms from objects and materials
Spontaneous generation
Early belief that some forms of life could arise from vital forces present in nonliving or decomposing matter (Ex. Flies from manure, etc)
Louis Pasteur-showed microbes caused fermentation and spoilage, and disproved spontaneous generation by use of swan neck flasks in his experiments
Aseptic technique
Joseph Lister (think listerene)-introduced aseptic technique in order...
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