Microbiology Study Guide

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Biology 2020 Microbiology
Study Guide for first exam

The history and scope of microbiology
1) What are the organisms/entities of study in the field of microbiology? 2) Who was the first human to publish extensive descriptions of microorganisms? 3) How did Louis Pasteur dismantle the theory of spontaneous generation in bacteria? 4) Who established microorganisms as the causative agent of infectious disease? How did he do this? What was the result of this body of work? 5) What are some of the advancements that were made possible once microorganisms were identified as the causative agents of infectious disease. 6) What are some of the future challenges in battling microorganism? Why are some of these challenges emerging?

The methods of microbiology
1) If you are viewing a specimen though a 60X objective lens on a compound microscope, what is the likely total magnification? 2) What type of lens does a microscope use to produce a magnified image? How does a lens produce a magnified image? 3) What are some ways to increase contrast in microscopy? 4) Define: simple stain, differential stain and special stain. 5) What are the steps in a gram stain?

6) How can staining aid in the diagnosis of TB?
7) In general, can viruses be imaged with a light microscope? 8) Are all bacteria microscopic (beyond the ability to see with the naked eye)?Prokaryotic structure and function
1) What are nanobacteria? What is some of the evidence for and against classifying them as a new type of bacteria? 2) What are some of the shapes that bacteria exist as?
3) Are all bacteria of a single species the exact same shape? 4) What are some of the ways in which prokaryotic and eukaryotic cells are different? 5) Explain some of the consequences of the small size of bacteria. Why do these consequences arise? 6) Label the basic structures of a bacterial cell.

7) What are...
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