Micro Lab Study Guide- Midterm

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• Essay about Lab Study Guides Study for Lab Test 1

  ...Study Guide to Help with Laboratory Exam 1 Microbiology 1214 A partial study guide only. Not intended to replace the review sheets or the “Know the following” sections on your labs. These slides are not presented in any particular order. Name this Procedure Identify the two types of bacteria present by shape and gram stain. In a gram stain what is the primary stain? The mordant? The counterstain? How does this differ from a simple stain? Identify the shape and gram stain of the bacteria present in this gram stain. Identify the shape and gram stain of the bacteria pictured. Think on this one! Name the gram stain and shape of the bacteria pictured. Name the genus of the organism pictured. Note the size when determining the organism. Identify this type of agar. We use this agar to check for _______. Name This Technique. What is its purpose? What type of hemolysis is pictured below? Identify the Following I go straight from the 4x objective to the 100 x objective? Why? Identify the Following Identify the staining procedure pictured. Identify what we are doing here. What type of plate would we be making using this technique? Name the Structure Pictured. What Settings Do We Use to Sterilize Bacteria Using this Instrument? What temperature do we use to sterilize? At what temperature does agar melt? At what temperature does agar solidify? At what temperature do we prefer to work...

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• Essay about Microbiology Lab Midterm Study Guide

  ... Review all chapters in your exercise manual beginning with the introduction INTRODUCTION: (Covered 9.4.14 II Week 1) Biosafety levels1: basic level of containment. Hand washing or wearing gloves 2: Appropriate for working with human body fluids. Autoclave, sharps containers, lab coats 3: appropriate for working with pathogens that can be transmitted via respiratory route. Self-closing, double doors and sealed windows 4: Highest level. Aerosol pathogens; pathogens with no vaccine/treatment. Separate building with separate ventilation and waste management systems Bacteria used in lab is classified as Class 1 by US Public Health Service/Biosafety Level 2 Review everything about the microscope and our original laboratory EXERCISE 1: USE AND CARE OF MICROSCOPE: (Covered 9.4.14 II Week 1) THE MICROSCOPE: Simple microscope early microscope; like a magnifying glass Brightfield compound microscope Most often used Base Bottom of microscope Stage Holds slide Arm Carries the microscope Body Tube Transmits the magnified image Condenser Lenses that concentrate light by focusing it into a cone Iris Diaphragm Controls the angle and size of light cone Objective lenses Magnify specimen Ocular/eyepiece lens: 10x Monocular One lens Binocular Two lens FOCUSING THE MICROSCOPE: Coarse adjustment Focuses low power objectives (4x and 10x) Fine adjustment Focusing with high-power (40x and 100x) Field of vision Area seen through microscope...

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• micro lab report Essay

  ...wrist showed bacteria showed gram positive and coccus morphology and finally, her right ear was gram negative and bacillus shape. Introduction: There are many reasons for identifying an unknown bacterium. The reasons range from medical purposes, such as determining if the unknown could cause ailments in living things or knowing what microorganisms are needed to make antibiotics to other purposes such as knowing the exact microorganism has to be used to make certain foods. We applied this method of gram staining in order to identify an unknown bacterium. Determining whether the samples are gram negative or positive helps in a clinical setting for patients in need of certain antibiotic. Procedures were followed as stated in the lab manual 3.6 I believe I will be able to determine cell wall art culture in my unknown bacteria. The four samples of the unknown bacteria were incubated at 37 degrees Celsius and isolated from the environment. The colonies were used in the Gram staining process to determine their morphology as well as determining the results. HDD Methods: In order to start the Gram staining technique, we made the smears and heat fixed the four samples of bacteria which were, my cheek and wrist and Brittany’s wrist and behind her right ear. Heat fixing allow the bacteria to adhere to the slides .The next step is to cover the slide with crystal violet stain for one minute. The crystal violet stain is used for gram...

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• Study guide Essay

  ...1. What nursing action is required b4 you measure fundal height= empty bladder full bladder make the fundal height higher. 2. What should a nurse do to prevent heat loss from evaporation= dry them up and remove the wet linen. 3. Child with cephalohematoma. What condition is associated with cephalohemetoma = jaundice 4. Why do we perform gestational age in a baby= to identify developmental level 5. What kind of exam do we perform to access for gestational age = ballot score 6. A baby has been circumcised a mother called the unit and complains that she saw a yellow crust on the penile area what do you tell the mother=Normal 7. You are teaching a mom how to use a bulb syringe what will you tell her to do= tilt babies head to the side and sanction the check 8. You are providing umbilical cord care, what will you do to provide this care= dye, open, dry, to prevent infection. 9. You have a patient who is breast feeding you want to prevent nipple trauma what will you teach= latching on, make sure the oriole is in the baby mouth and the baby is sucking onto it. And the baby is not sucking the nipple. 10. When babies have jaundice and are placed on a phototherapy why should we make sure that they have fluid and they get fed= prevent dehydration, hypoglycemia and promote growth 11. A neonate that was born 4hours after delivery mother is diabetic and some of the signs and symptoms is that the baby is jittery = hypoglycemia, check blood sugar and feed them 12. A woman who...

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• Essay about Micro lab quiz 2

  ...Microbiology Lab Quiz #2 Study Guide Exercise 9 -- The Gram Stain Compare and contrast simple and differential staining techniques. Simple Staining Simple staining is useful in determining the basic morphology of an organism. Simple staining involves only one reagent i.e. crystal violet, basic fuchsin, or methylene blue. Simple dyes used to stain bacteria have a positive charge cationic (+), therefore, they are attracted to the bacteria that are usually negatively charged anionic (-) Depends on the fact that bacteria differ chemically from their surroundings and thus can be stained with their environments Simple stains are used to provide contrast between specimen and background Differential Staining Bacteria react differently to the multiple reagents that are used i.e. Gram Stain = Crystal violet, Grams Iodine, 95% ethyl alcohol, and safranin. i.e. Acid-fast stain Can distinguish betweeen 2 types of bacteria because bacteria differ from one another chemically and physically to react differently to a given staining procedure. State the purpose of the Gram stain. The Gram stain separates bacteria into two major groups: Gram positives that stain purple, and Gram negatives that stain pink or red. Primary Stain = Crystal violet; Mordant = Grams Iodine; Decolorizer = 95% ethyl alcohol; Counterstain = Safranin. Perform and interpret a Gram stain. Gram positive = stains purple Gram negative = stains pink or red...

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• Micro Unknown Lab Report Essay

  ...| Gram Negative Unknown | Biology 3444-006 | | Lena Wallace | 11/7/2011 | | Abstract: The purpose of this lab was to identify an unknown bacteria culture using differential tests. The identification of the unknown culture was accomplished by identifying the bacteria based on its specific metabolic characteristics and morphology. It is suggested that culture 11 is a sample of Enterobacter aerogenes. Introduction: This experiment was centered on metabolic and biochemical testing procedures. The rationale of performing these tests was to distinguish six different microbes from one another and to compare how their metabolic and biochemical processes differ from species to species to determine the unknown sample. The tests included: Triple sugar iron agar (TSAI), the Sulfide Indole Mobility (SIM) test, Glucose fermentation, the Methyl Red test, the Voges-Proskauer test, Citrate test, the Urease Test, and finally the Gelatin test. The microbes that were tested during this lab were: Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, and Salmonella typhimurium. The sample labeled #11 could have been any of the six microbes. A gram stain was performed to assess the shape and other characteristics of the bacteria, and to ensure that there was no gram positive contamination. Gram positive cells have a thick outer peptidoglycan layer that traps the crystal...

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• Ap Micro Study Guide Essay

  ...STUDY GUIDE COSTS OF PRODUCTION: Total Physical Product (TP)- total output or quantity produced Marginal Product (MP)- the additional output generated by additional inputs (workers). Average Product (AP)- the output per unit of input. Stage 1: Increasing Marginal Returns Stage 2: Decreasing Marginal Returns Stage 3: Negative Marginal Returns TC- Total Costs: Sum of Fixed and Variable Costs VC- Variable Costs: Costs for variable resources that do change with the amount produced. FC- Fixed Costs: Costs for fixed resources that don't change with the amount produced. AFC AVC ATC MC MC = Change in Total Cost/Change in Quantity ATC = Total Costs/Quantity AVC= Variable Costs/Quantity AFC= Fixed Costs/Quantity Long-Run ATC: Economies Of Scale Constant Returns To Scale Diseconomies of Scale ATC II. PERFECT COMPETITION: Characteristics: Many Small Firms Identical Products (Perfect Substitutes) Easy for firms to enter and exit the industry Seller has no need to advertise Firms are price takers: the seller has no control over price Firm and Industry in Short-Run Making Profit: Firm and Industry in Short-Run Making Loss: MC AVC ATC MC AVC ATC Firm and Industry in Long-Run Equilibrium: ATC MC MR=D How Economic Profit and Loss disappear in the Long-Run: MR=D ATC MC TC = TR III. MONOPOLIES: Characteristics: Single Seller: One...

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• Brandy Skelton Micro Lab 5 Essay

  ...Brandy Skelton 2-18-15 Lab 5 Microbiology Laboratory BIO 2921-90 Table 1: Experiment 1 Staining Observations Stain Used: Crystal Violet Observations: All bacteria become directly stained and become purple in appearance. 1. What difference would you expect to observe when changing the magnification from 100X to 200X? I would expect to see a clearer picture of the bacteria/organisms. What changes would you expect to observe when changing magnification from 200X to 600X? I would expect to see a difference in the shape, size, and ease of identification of the bacteria and organisms as well as a change in clarity. 2. How does crystal violet enhance the visualization of bacterial structure(s)? Crystal violet enhances the visualization of bacterial structures by making gram positive organisms appear purple/brown, and gram negative appear red. Table 2: Experiment 2 Staining Observations Stain Used: Nigrosin Observations: Organisms appear as color free outlines against the background of the slide. 1. How does nigrosine work in the staining process? What components does nigrosin stain? Since the surface of most bacterial cells is negatively charged, the cell surface repels the stain. The glass of the slide will stain, but the bacterial cells will not. The bacteria will show up as clear spots against a dark background. 2. What physical or visual characteristics did you notice when performing the negative stain that you were not able to visualize...

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