Glucose Oxidation by Gluconobacter Oxydans: Characterization in Shaking Flasks
Appl Microbiol Biotechnol (2003) 62:92–98
DOI 10.1007/s00253-003-1222-x
ORIGINAL PAPER
M. Silberbach · B. Maier · M. Zimmermann · J. Büchs
Glucose oxidation by Gluconobacter oxydans: characterization in shaking-flasks, scale-up and optimization of the pH profile
Received: 20 September 2002 / Revised: 3 December 2002 / Accepted: 6 December 2002 / Published online: 26 February 2003
Springer-Verlag 2003
Abstract In this study, the advantage of a novel measuring device for the online determination of oxygen and carbon dioxide transfer rates in shaking-flasks is reported for glucose oxidation by Gluconobacter oxydans.
In this fermentation process, this device was used for the characterization of the oxidation pattern of different strains. G. oxydans NCIMB 8084 forms 2,5-diketogluconate from d-glucose in a multi-stage process via three different membrane-bound dehydrogenases. This strain was chosen for a scale-up of the process from shakingflasks to a 2-l stirred vessel. An enhancement of 2,5diketogluconate production was realized by controlling the pH at different levels during the fermentation.
Introduction
A main characteristic of Gluconobacter sp. is its ability to rapidly oxidize many organic compounds to the corresponding acids and ketones (Asai et al. 1968). The accumulation of products in the medium was exploited to establish several industrial processes using strains of this genus, e.g. the production of l-sorbose from d-sorbitol, the production of dihydroxyacetone from glycerol and the production of d-gluconate from d-glucose (Lusta and
Reshetilov 1998; Macauley et al. 2001). d-Glucose can be oxidized by two different metabolic pathways: one includes uptake, intracellular oxidation and further dissimilation via the pentose phosphate pathway; and the other consists of the direct oxidation by membrane-bound pyrroloquinoline quinone (PQQ)-dependent glucose deM. Silberbach · B. Maier · J. Büchs ())
Department of
DOI 10.1007/s00253-003-1222-x
ORIGINAL PAPER
M. Silberbach · B. Maier · M. Zimmermann · J. Büchs
Glucose oxidation by Gluconobacter oxydans: characterization in shaking-flasks, scale-up and optimization of the pH profile
Received: 20 September 2002 / Revised: 3 December 2002 / Accepted: 6 December 2002 / Published online: 26 February 2003
Springer-Verlag 2003
Abstract In this study, the advantage of a novel measuring device for the online determination of oxygen and carbon dioxide transfer rates in shaking-flasks is reported for glucose oxidation by Gluconobacter oxydans.
In this fermentation process, this device was used for the characterization of the oxidation pattern of different strains. G. oxydans NCIMB 8084 forms 2,5-diketogluconate from d-glucose in a multi-stage process via three different membrane-bound dehydrogenases. This strain was chosen for a scale-up of the process from shakingflasks to a 2-l stirred vessel. An enhancement of 2,5diketogluconate production was realized by controlling the pH at different levels during the fermentation.
Introduction
A main characteristic of Gluconobacter sp. is its ability to rapidly oxidize many organic compounds to the corresponding acids and ketones (Asai et al. 1968). The accumulation of products in the medium was exploited to establish several industrial processes using strains of this genus, e.g. the production of l-sorbose from d-sorbitol, the production of dihydroxyacetone from glycerol and the production of d-gluconate from d-glucose (Lusta and
Reshetilov 1998; Macauley et al. 2001). d-Glucose can be oxidized by two different metabolic pathways: one includes uptake, intracellular oxidation and further dissimilation via the pentose phosphate pathway; and the other consists of the direct oxidation by membrane-bound pyrroloquinoline quinone (PQQ)-dependent glucose deM. Silberbach · B. Maier · J. Büchs ())
Department of
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