Exercise 11 Blood Analysis

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Exercise 11: Blood Analysis: Activity 1: Hematocrit Determination Lab Report Pre-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly. 1. Hematocrit
You correctly answered: b. of 40 means that 40% of the volume of blood consists of RBCs. 2. A buffy coat layer
You correctly answered: d. is all of the above.
3. The diagnosis of anemia indicates
You correctly answered: a. a lower-than-normal hematocrit.
4. Polycythemia refers to
You correctly answered: c. a significant increase in RBCs.

02/03/13

page 1

Experiment Results
Predict Question:
Predict Question: Predict how the hematocrits of the patients living in Denver, Colorado (approximately one mile above sea level), will compare with the hematocrit levels of the patients living in Boston, Massachusetts (at sea level). Your answer : c. The hematocrits of the Denver residents will be higher than those of the Boston residents. Stop & Think Questions:

Why would the hemoglobin levels of an anemic patient be lower than the hemoglobin levels of a normal, healthy individual? You correctly answered: c. Because hemoglobin resides in RBCs, you would anticipate a low hematocrit level to coincide with a low hemoglobin level.

Experiment Data:
Blood sample
1
2
3
4
5
6

Height of column of
blood
100 mm
100 mm
100 mm
100 mm
100 mm
100 mm

Height of red blood cell
layer
48 mm
44 mm
55 mm
53 mm
19 mm
32 mm

02/03/13

Height of buffy coat
(white blood cells)
1 mm
1 mm
1 mm
1 mm
0.5 mm
1 mm

page 2

Hematocrit

% WBC

48
44
55
53
19
32

1
1
1
1
0.5
1

Post-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly. 1. Anemia refers to
You correctly answered: c. a lower-than-normal hematocrit.
2. To complete the blood test to measure hematocrit, you must seal the blood-containing capillary tubes on one end with a clay material so that
You correctly answered: d. the blood sample can be centrifuged without having the blood spray out of the tube. 3. Possible causes of polycythemia include
You correctly answered: b. living at high altitudes.
4. You would expect anemia to develop in a person
You correctly answered: c. who has abnormally shaped hemoglobin in their RBCs.

02/03/13

page 3

Review Sheet Results
1. List the hematocrits for the healthy male (sample 1) and female (sample 2) living in Boston (at sea level) and indicate whether they are normal or whether they indicate anemia or polycythemia. Your answer:

Sample 1=48, wnl,
Sample 2=44, wnl,
2. Describe the difference between the hematocrits for the male and female living in Boston. Why does this difference between the sexes exist?
Your answer:
Male HCT higher due to increased RBC production as result of testoterone, epo. 3. List the hematocrits for the healthy male and female living in Denver (approximately one mile above sea level) and indicate whether they are normal or whether they indicate anemia or polycythemia. Your answer:

Sample 3= 55, higher, indicates RBC polycythemia
Sample 4=53, higher, indicates RBC pollycythemia
4. How did the hematocrit levels of the Denver residents differ from those of the Boston residents? Why? How well did the results compare with your prediction?
Your answer:
HCT increase to compensate for the rarefied air at higher altitudes as seen in Denver. 5. Describe how the kidneys respond to a chronic decrease in oxygen and what effect this has on hematocrit levels. Your answer:

Hypoxia triggers EPO release/production which in turn stimulates RBC production in marrow. 6. List the hematocrit for the male with aplastic anemia (sample 5) and indicate whether it is normal or abnormal. Explain your response.

Your answer:
Sample 5 HCT=19. Significant decrease in HCT, indicating anemia, abnormal. This is expected as aplastic anemia is a condtion that results from the suppression of RBC production in the marrow. 7. List the hematocrit for the female with iron-deficiency anemia...
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