Exercise 1: Cell Transport Mechanisms and Permeability Worksheet
Student instructions: Follow the step-by-step instructions for this exercise found in your lab manual and record your answers in the spaces below. Submit this completed document by the assignment due date found in the Syllabus. Rename this document to include your first and last name prior to submitting, e.g. Exercise1_JohnSmith.doc. Please make sure that your answers are typed in RED.
Grading: True/False, Multiple-Choice, and Fill-in-the-blank type questions will be worth 1 point each whereas Short-answer type questions will be worth 2 points each. This lab will be worth a total of 45 points but will be converted to a percentage grade when registered in your “Gradebook”.
|ACTIVITY 1: Simulating Dialysis (Simple Diffusion) |Answers | |The molecular weight of urea is approximately 60. Which of the membranes can it pass through? |D | |50 MWCO membrane | | |100 MWCO membrane | | |200 MWCO membrane | | |Both the 100 and 200 MWCO membranes | | |True or False: A solution containing glucose will diffuse faster through a 200 MWCO membrane if it|True | |is heated. | | |The molecular weight of carbon C is 12; the molecular weight of hydrogen (H) is 1; the molecular |6 x 12 = 72, 6 x 16 = 96, 12 x 1 = 12 | |weight of oxygen (O) is 16. The chemical formula of glucose is: C6H12O6 . Using some simple | | |multiplication and addition, determine the molecular weight of one molecule of glucose. |Total = 180 | |Describe two variables that affect the rate of diffusion. |MWCO of the membrane, molecular weight of the ion or molecule | |Why do you think that the urea was not able to diffuse through the 20 MWCO membrane? |Urea will not diffuse through the 20 MWCO membrane because it | | |doesn’t ionize | |Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. |Glucose will diffuse through 200 MWCO membrane however albumin | | |will not | |Put the following in order from smallest to largest molecular weight: glucose, sodium chloride, |Sodium Chloride, Urea, Glucose, Albumin | |albumin, and urea. | | |ACTIVITY 2: Simulated Facilitated Diffusion |Answers | |True or False: The facilitated...
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