Ex1 Lab Report

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Alejandra Trujillo (3378033)
MCB2000L (M: 11am – 1:40pm)
Jose Roble
January 6, 2014

Title: Experiment 1: Microscopy of Living Microbes

Purpose: To prepare students to familiarize themselves with the lab equipment such as the microscope, square glass, cover slips, etc.; to facilitate students to observe the pond water; and enable them to discern and identify the microbes as bacteria, algae, fungi, or protozoa within the pond water. Additionally, this lab experiment will permit students to observe and distinguish the yeast suspension from the other microbes.

Data/Results: (Attached)

Conclusion:
The student acquired the fundamental skills in which to properly handle lab equipment and execute an experiment. By examining the pond water with the 40X lens, the student was able to distinguish and identify the protozoa with cilia: colpidium, which was recognized by its unique oval shape, resembling a peanut, with small hair-like particles comprising the entire surface, and the vascular plant, which was discrete due to its array of composition of cells. By examining the pond water with the 100X lens, the student was able to differentiate and classify 1) the cyanobacteria: nostoc due to its distinctive S shape and beads with surrounding cells, 2) two nematodes (phylum nematoda) parallel to one another (the nematodes appeared as slender worms, colorless, and encompassed of cells), and 3) the cyanobacteria: anabaena, which was blue-green in its feature with long filaments of cells (resembling a seaweed structure). By examining the yeast suspension with the 40X lens, the student observed millions of budding yeast cell structures. The student was able to discern between the various structures of pond water microorganisms, such as cilia and algae, and the yeast suspension cellular material.

Questions:
1. What is Brownian motion and why is it generally seen when bacteria are observed in liquid media? Some bacteria, perhaps many in your presentation did...
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