Enzyme Lab Report

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Influence of Concentration on the Activity of Amylase

Title

Influence of Concentration on the Activity of Amylase

Abstract

Does more or less concentration speed up the reaction rate of amylase in starch? In this experiment diluted solutions of amylase were created and then tested using a starch solution, I2KI for reaction times. The answer to the question was proved to be that more concentration of amylase speeds up the reaction time.

Introduction

The enzyme, amylase is found in the saliva of most animals and in humans. Amylase hydrolyzes starch, a plant's reservation of carbohydrates. Amylase causes a chemical reaction in the polysaccharide starch that breaks down the glucose molecules into maltose. When diluted with a solution of I2KI, starch will change to a dark purple while doing the same to maltose, there would be no color change. The question posed, does more or less concentration speed up the reaction rate? Group 11 predicts that the more concentrated solution of amylase (1>5), the reaction rate will be faster; therefore test tube 1 will have the quickest reaction rate. The rate of disappearance of starch in amylase can be shown by diluting amylase in a 1:1 ratio but using Tube 1 to create Tube 2, Tube 2 (a mix of 1 and 2) to create Tube 3 and so on for 5 Tubes.

Materials

test tube rack
10 standard test tubes
wax pencil
test plate
flask of distilled water
beaker of distilled water
5mL graduated cylinder
1 calibrated 1mL pipette
2 calibrated 5mL pipette
disposable pasteur pipettes
pipette bulb
buffer solution
I2KI solution
1% starch solution
1% amylase solution

Methods

The lab group prepared amylase dilution in the first set of test tubes (1-5). 5mL amylase was added to a test tube already containing 5mL distilled water creating the first tube to have .5% amylase. Next 5mL of amylase from test tube 1 was added to the 5mL of distilled water in test tube 2, giving test tube 2 a dilution of .25%. This was...
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