Cell Membranes lab report

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BioLab3
Lab Report 7
Cellular Respiration Answer Key

Student Name:

I. The ATP Cycle

Define the following terms:
Autotroph

Heterotroph

Aerobic respiration

Anaerobic respiration

Complete the chart below:

Letter
Defining Term
A

B

C

D

II. Anaerobic Respiration

Define the following terms:
Alcoholic fermentation

EXERCISE 1 – Alcoholic fermentation

At intervals of 20, 40, and 60 minutes, the tubes are removed. Record the volume of gas produced in each fermentation tube. Each tube is graduated in tenths of a milliliter. (HINT: Look at the amount of gas, not the level of the liquid.) Tube

Solution
Gas Volume in ml after:

20 min.
40 min.
60 min.
1
10% glucose + yeast

2
1% starch + yeast

3
1% starch + yeast + amylase

What gas accumulated in the calibrated portion of the fermentation tube?

What metabolic pathway is utilized by yeast cells?

Why was amylase added to the third test tube?

Did respiration occur in the beaker containing yeast and starch? Why?

III. Aerobic Respiration

Define the following terms:
Glycolysis

Kreb’s or citric acid cycle

Electron transport phosphorylation

A.Carbon Dioxide Production

EXERCISE 2 – Carbon dioxide production in germinating seeds

Record the experimental results below.

Bean Seeds
Indicator color
CO2
+ or -
Germinating

Germ-boiled

Ungerminated

What color is the phenol red solution in the stock bottle?

What does this indicate about the pH of the phenol red solution in the stock bottle?

What color was the solution in the beaker after exhaling into the solution?

Explain the color change.

Which laboratory setup produced carbon dioxide?

B.Oxygen Consumption

EXERCISE 3 – Measuring metabolic rate

Record the time measurement for the soap film to travel 2 ml of the pipette.

Oxygen Consumed (ml)
Time (seconds)
Test 1
2

Test 2
2

Test 3
2

Average
2

Summary: Calculating Metabolic Rate
1.
Mass of rodent (g):

2.
Surface area of rodent in square meters (m2):

3.
Average time to consume 2 ml of oxygen from Table 7.3 (sec):

4.
Oxygen consumption by the rodent (l/hr):
Calculation = 2ml/45.67 multiplied by 3600/1000

5.
Calculated oxygen consumption of the rodent (l/hr/m2):
Calculation = .1576/.0139

6.
Calculated metabolic rate of rodent (Cal/hr/m2):
Calculation = 11.34 X 4.8

IV. The Heat Balance

Define the following term:
Endotherm

Does the logarithmic graph in your lab indicate the gerbil’s metabolic rate is faster or slower than that of a human?

Suggest explanations for the difference in rate.

EXERCISE 4 – Estimate basal metabolic rate

Record your height, age, weight, and sex. Answers will vary Height

Weight

Age

Sex

Using the formula in your lab, calculate your BMR at your current age, and in 10, 20, 30 and 40 years.

Current age
+10
+20
+30
+40
Age

BMR

Assuming that your calorie intake and activity level do not change, what happens to your BMR as you age?

Indicate how the following factors affect BMR.
Age

Gender

Exercise level

Body temperature

Hormones

Body surface area

Nutritional state

External temperature

Summary Questions

1. Compare aerobic and anaerobic respiration using the following table. Type of respiration
ATP Produced
End Products
Aerobic respiration

Anaerobic respiration

2. What human foods are products of fermentation?

3. Explain why the sun is the ultimate source of energy for living organisms.

4. Describe the relationship between photosynthesis and respiration. Include an equation that demonstrates this relationship.

5. In what organelle is pyruvate converted into ATP?

6. Define metabolism.

7. What is the connection between Calories and cellular...
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