Observation of Prokaryotic and Eukaryotic
Many of the cells and organisms that you will be studying are at the lower limits of visibility of light microscopes; therefore, it is extremely important that you attain critical lighting and focussing. It is also important to handle the microscope competently to avoid damaging either the microscope or the preparation you are studying. Even students who have previously used microscopes should read the instructions carefully.
to the following web site:
http://salinella.bio.uottawa.ca/biolabo/ (you can try it from home). Under Microscopy you will find links to pages that describe both type of microscopes you will use this semester, as well as how to set up and use them. It is strongly recommended that you visit these pages prior to attending your first lab.
Image J / Qcapture
Although you can make all your observations by watching directly through the oculars, it also can be done on the computer screen using the digital camera attached to each microscope. For that, you will use the Image J program together with a capture plugin called Qcapture. Visit the lab website to learn how to use Image J (link on the homepage). All observations can be made on your computer screen or in the oculars. Each method has its advantages and drawbacks; you will have to choose which one it more appropriate (or the one you prefer):
Wider field of view
Can share observation with others
More comfortable for users
Take pictures while observing
Lab1 ‐ Microscopy
The Compound Microscope
On the Guide Biolabo page click on the CX41 Compound Microscope link then on Parts and Function. This will bring up a labelled line diagram of your microscope. Familiarize yourself with the various components shown in this figure. Then, click on Setup and Bright field alignment in order to know how to use and handle the microscope.
Now, locate your compound microscope in the cupboard below the sink of your workstation. Place it on the counter between the computer and the end of the counter. Be sure that whenever you transport the microscope, it is always kept upright; the ocular lens will fall out if the scope is tilted or swung.
Even though you don’t need the dissecting microscope right now, take it out of the cupboard and install it beside the compound microscope. Connect one firewire cable to each of the cameras installed on top of the microscopes. This way, everything is setup for further observations both on your computer screen and through the oculars.
Parts of the compound microscope
The microscope consists of a system of lenses, a light source, and a geared mechanism for adjusting the distance between the lens system and object being observed. There are a number of important components and it is essential that you be able to identify them and understand their function before you can proceed. By going through the different modules in Biolabo and using the microscope you will develop a competency for bright field microscopy.
Identify the following components using Biolabo (Parts and functions figure) and your microscope:
REVOLVING NOSEPIECE: Supports the various objectives ‐ You will only use the 4x, 10x and 40x objectives in the BIO1140 labs (not the 100x). STAGE: Supports the specimen being observed. A system of knobs on the side of the stage allows you to move the ...