Activity 4 Assessing Fat Digestion by Pancreatic Lipase and the Action of Bile

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Digestion Lab Notebook Due Sunday at Midnight CST Week 6, Worth 100 points, pg. 105 Questions: 60 points
Data/Results: 15 points
Summarizing activities: 25 points
Name:
SC 245 Lab
Date:
Exercise 8 Chemical and Physical Processes of Digestion, pg. 105 Introduction: In this lab, I will learn how the complex digestive system breaks down substrates such as fats, proteins and carbohydrates. Activity 1: Assessing Starch Digestion by Salivary Amylase

Data:

Chart 1 – Salivary Amylase Digestion of Starch

Tube #1234

AdditivesAmylase, StarchAmylase, Starch Amylase, DI Water DI Water, Starch

pH 7.0 bufferpH 7.0 bufferpH 7.0 buffer pH 7.0 buffer

Incubation Cond. Boil, Inc. 37 deg.C 37 degrees C 37 degrees C 37 degrees C

for 60 minutes

IKI Test:

Benedict's Test:

Chart 1 – Salivary Amylase Digestion of Starch (5,6, 7)

Tube #567

AdditivesDI Water, Maltose Amylase, Starch Amylase, Starch

pH 7.0 buffer pH 2.0 buffer pH 9.0 buffer

Incubation Cond. 37 degrees C 37 degrees C 37 degrees C

IKI Test:Insert data

Benedict's Test:Insert data

Questions: Please answer the questions in complete sentences and explain your answers.

1. What ph allowed the highest amylase activity and why? Please explain. 2. What effect does boiling have on enzyme activity?
3. Would the amylase present in the mouth be active in the stomach? Please explain your answer. 4. Does amylase digest peptidase?
5. Which tubes indicate that deionized water did not contain contaminating starch or maltose? Summary:

In this Activity, I learned/observed that: Please write at least 2-3 sentences of what you learned or observed in complete sentences.

Activity 2: Assessing Cellulose Digestion

Data:

Chart 2 - Enzyme Digestion of Starch and Cellulose

Tube #1234

AdditivesAmylase, StarchAmylase, Starch Amylase, Glucose Amylase, Cellulose

pH 7.0 bufferpH 7.0 buffer pH 7.0 buffer pH 7.0 buffer

Incubation Cond. Freeze, Inc.37 degrees C37 degrees C 37 degrees C

37 deg C for 60 min 60 min. 60 min. 60 min.

IKI Test

Benedict's Test

Chart 2 Enzyme Digestion of Starch and Cellulose (Tubes 5, 6, 7)

Tube #567

AdditivesAmylase, Cellulose Peptidase, Starch Bacteria, Cellulose

DI Water pH 7.0 buffer pH 7.0 buffer

Incubation Cond. 37 degrees C 37 degrees C 37 degrees C

60 min. 60 min. 60 min.

IKI TestInsert data

Benedict's TestInsert data

Questions: Please answer the questions in complete sentences and explain your answers.

1. Which tubes showed that starch or cellulose was still present?

2. Which tubes tested positive for the presence of reducing sugars?

3. What was the effect of freezing tube 1?

4. Does amylase use cellulose as a substrate? (Hint: Look at the results for Tube 4).

5. What effect did the addition of bacteria have on the digestion of cellulose?

Summary:

In this Activity, I learned/observed that: Please write at least 2-3 sentences of what you learned or observed in complete sentences.

Activity 3: Assessing Protein Digestion by Pepsin

Data:

Chart 3 - Pepsin Digestion of Protein

Tube #1234

AdditivesPepsin, BAPNAPepsin, BAPNA Pepsin, DI Water DI Water, BAPNA

pH 2.0 bufferpH 2.0 buffer pH 2.0 buffer pH 2.0 buffer

Incubation...
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